蚕梅方干预炎癌转化模式小鼠肠组织蛋白质组学差异表达研究  

Study on Canmei Formula intervening proteomic differential expression in intestinal tissue of inflammatory carcinoma mice

作　　者：徐祎敏 郭翠 李园 韩馨悦 奚建强 郝五四 刘晓强 谢国群[1] 郭晓冬[1] 付晓伶 XU Yi-min;GUO Cui;LI Yuan;HAN Xin-yue;XI Jian-qiang;HAO Wu-si;LIU Xiao-qiang;XIE Guo-qun;GUO Xiao-dong;FU Xiao-ling(The Second Department of Oncology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;Department of Infection,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Natural Medicine Preparation Room,National Pharmaceutical Engineering Research Center,Shanghai 201203,China;Department of Pain,Shibei Hospital,Jing'an District,Shanghai 200435,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院肿瘤二科,上海200437 [2]辽宁中医药大学,沈阳110847 [3]上海中医药大学附属岳阳中西医结合医院院感科,上海200437 [4]药物制剂国家工程研究中心天然药物制剂室,上海201203 [5]上海市静安区市北医院疼痛科,上海200435

出　　处：《中华中医药杂志》2022年第9期5370-5374,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市科技委员会资助项目(No.21Y11922300);上海市体育科技备战攻关计划项目(No.20J019);上海中医药大学附属岳阳中西医结合医院重点项目(No.2019YYZ08)。

摘　　要：目的:应用非标记定量(label-free)蛋白质组学技术,探究蚕梅方对炎癌转化模式小鼠肠组织蛋白质差异表达的影响。方法:从对照组、结直肠腺瘤模型组(模型组)、蚕梅方水煎醇沉给药组(CMF-A组)小鼠肠组织中提取蛋白,进行label-free蛋白质组学研究及分子对接分析。结果:蛋白质组学研究共鉴定出3 456个蛋白质,与模型组比较,CMF-A组差异蛋白有515个,与对照组比较,模型组差异蛋白有15个,其中上调蛋白6个,下调蛋白9个。模型组与对照组比较有差异而蚕梅方可使差异趋势逆转的蛋白有1个,即线粒体输入受体亚基TOM20同源物(TOMM20)。GO分析发现差异表达蛋白主要参与ATP代谢等生物过程,KEGG分析提示差异蛋白共涉及产热作用通路等37条信号通路,分子对接显示麦角胺等活性成分与TOMM20具有良好相互作用。结论:蚕梅方可能通过下调TOMM20的表达,通过ATP代谢等多个生物过程,作用于多条信号通路,达到对炎癌转化的防治作用。Objective: To investigate the effects of Canmei Formula on the proteomic differential expression in the intestinal tissue of mice with inflammatory cancer transformation model by label-free proteomics technology. Methods: Proteins were extracted from the intestinal tissues of mice in the normal group, the colorectal adenoma model group, and the Canmei Formula and alcohol precipitation administration group(CMF-A) for label-free proteomics research and molecular docking analysis. Results:A total of 3 456 proteins were identified in proteomics research. Compared with the model group, the CMF-A group had 515 different proteins. Compared with the normal group, there were 15 different proteins in the model group, including 6 upregulated proteins and 9 downregulated proteins. There was a difference between the model group and the normal group, and Canmei Formula could reverse the trend of the difference. There was one protein, that is, the mitochondrial import receptor subunit TOM20 homolog(TOMM20). GO analysis found that differentially expressed proteins were mainly involved in biological processes such as ATP metabolism. KEGG analysis revealed that the differential proteins involved 37 signaling pathways such as thermogenic pathway.Molecular docking showed that ergotamine and other active components had a good interaction with TOMM20. Conclusion:Canmei Formula may reduce the expression of TOMM20 and act on multiple signal pathways through multiple biological processes such as ATP metabolism to achieve the prevention and treatment of inflammatory cancer transformation.

关 键 词：蚕梅方 炎癌转化 TOMM20 label-free蛋白质组学 生物信息学分析 

分 类 号：R285.5[医药卫生—中药学]