Mce转运体ATP酶过表达对分枝杆菌转化植物甾醇的影响  

Effect of Over-Expression of Mce Transporter ATPase on Conversion of Phytosterols by Mycobacterium sp.LZ2

作　　者：姚莹莹 王靖 徐倩倩 周秀玲[1] 张扬[1] YAO Yingying;WANG Jing;XU Qianqian;ZHOU Xiuling;ZHANG Yang(College of Life Science,Liaocheng University,Liaocheng 252059,China)

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《化学与生物工程》2022年第11期1-7,12,共8页Chemistry & Bioengineering

基　　金：国家自然科学基金项目(32100065);山东省自然科学基金项目(ZR2018QC002)。

摘　　要：以分枝杆菌Mycobacterium sp.LZ2(M.sp.LZ2)的Mce转运体ATP酶(LzMceG)为研究对象,在获取lzmceG基因全长序列的基础上对其进行结构域、系统发育等生物信息学分析,利用表达型质粒pMV261构建LzMceG过表达菌株M.sp.LZ2/pMV261-lzmG。转录水平分析表明,MceG能够响应植物甾醇的代谢,对植物甾醇转化具有重要作用。菌株生长和植物甾醇转化得率分析结果表明,M.sp.LZ2/pMV261-lzmG的最高比生长速率达到0.045 h^(-1),最高植物甾醇转化得率达到81.32%。表明,LzMceG的过表达有利于M.sp.LZ2的生长和植物甾醇摄取,可提高对植物甾醇的转化能力。Taking Mce transporter ATPase(LzMceG)of Mycobacterium sp.LZ2(M.sp.LZ2)as the research object,we carried out the bioinformatics analysis,such as the structure domain and phylogeny,on the basis of the full-length sequence of the lzmceG gene.Moreover,we constructed the LzMceG over-expressing strain M.sp.LZ2/pMV261-lzmG by using the expression plasmid pMV261.Transcription levels analysis show that the MceG can respond to the metabolism of phytosterols and plays an important role in phytosterols conversion.The analysis results of the growth of strain and conversion yield of phytosterols show that the highest specific growth rate of M.sp.LZ2/pMV261-lzmG can reach 0.045 h^(-1),and the highest conversion yield can reach 81.32%.The study indicates that the over-expression of LzMceG can facilitate the growth of M.sp.LZ2 and uptake of phytosterols,and can improve the conversion capacity of phytosterols.

关 键 词：ABC转运体ATP酶 分枝杆菌LZ2 植物甾醇 生物转化 

分 类 号：TQ463.54[化学工程—制药化工] R977.1[医药卫生—药品]