一种水溶性超分子荧光探针对农药缩节胺的识别  

A Turn-on Supramolecular Fluorescent Probe for Sensing Mepiquat Chloride

作　　者：陈丽霞[1] 宋桂先 杨梅[1] 黄英[1,3] 卫钢 陶朱 CHEN Lixia;SONG Guixian;YANG Mei;HUANG Ying;WEI Gang;TAO Zhu(Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province,Guizhou University,Guiyang 550025,China;College of ShenQi Ethinc Medicine,Guizhou Medical University,Guiyang 550005,China;The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China,Guizhou University,Guiyang 550025,China;Commonwealth Scientific and Industrial Research Organization(CSIRO)Manufacturing,PO Box 218,NSW 2070,Australia)

机构地区：[1]贵州大学贵州省大环化学及超分子化学重点实验室,贵州贵阳550025 [2]贵州医科大学神奇民族医药学院,贵州贵阳550005 [3]贵州大学西南药用生物资源教育部工程研究中心,贵州贵阳550025 [4]澳大利亚联邦科学与工业组织(CSIRO),新南威尔士州2070

出　　处：《贵州大学学报（自然科学版）》2022年第6期43-48,共6页Journal of Guizhou University:Natural Sciences

基　　金：国家自然科学基金资助项目(21761007,21871064);贵州省优秀青年科技人才培养计划资助项目([2017] 5636);贵州省普通高等学校科技拔尖人才支持计划资助项目(黔教合KY字[2016]059);教育部春晖计划资助项目(Z2015006)。

摘　　要：八元瓜环(Q[8])能使碱性黄T(thioflavin T,ThT)荧光增强,在荧光探针(Q[8]/ThT)中加入缩节胺(mepiquat chloride,MC)后荧光强度又逐渐降低,因此,利用超分子配合物Q[8]/ThT的荧光“开-关”效应,设计了一种超分子荧光探针(Q[8]/ThT),实现了MC的快速检测。研究结果表明,该探针在pH=7的Tris-HCl溶液中对MC具有很好的识别能力,且在一定浓度范围呈线性关系,其检出限为3.6×10^(-8) mol/L。此外,通过核磁共振波谱(1H NMR)和等温滴定量热法(isothermal titration calorimetry,ITC)考察了探针对MC的识别机理,推测出MC的加入与超分子荧光探针(Q[8]/ThT)中Q[8]的端口发生了主客体相互作用,即Q[8]、ThT和MC形成了2∶2∶2的作用模式。Cucurbit[8]uril(Q[8])could increase the fluorescence of thioflavin T(ThT),the intensity of which is gradually decreased after adding mepiquat chloride(MC)to the fluorescent probe(Q[8]/ThT).Thus,a kind of supramolecular fluorescent probe(Q[8]/ThT)was designed through the fluorescence“on-off”effect of the supramolecular complex of Q[8]/ThT,which could realize the rapid detection of MC.The research results of the study showed that the fluorescent probe had a good ability to recognize MC in a Tris-HCl solution of pH=7,and had a linear relationship within a certain concentration range,with a detection limit of 3.6×10^(-8) mol/L.Furthermore,the recognition mechanism of the probe to MC was investigated through 1H NMR spectroscopy and isothermal titration calorimetry(ITC),and it was inferred that the addition of MC had a host-guest interaction with the two open portals of Q[8]of the supramolecular fluorescent probe(Q[8]/ThT),namely,Q[8],ThT,and MC formed a 2∶2∶2 mode of action.

关 键 词：八元瓜环 碱性黄T 缩节胺 超分子荧光探针 

分 类 号：O657.3[理学—分析化学]