地骨皮药材饮片和标准汤剂的定量检测及UPLC特征图谱的建立  

Quantitative Detection and UPLC Characteristic Chromatogram of Raw Herb,Decoction Pieces and Standard Decoction of Lycii Cortex

作　　者：史紫娟 曾杉 林碧珊 高永坚 刘勇 梁思韵 区淑蕴 SHI Zi-juan;ZENG Shan;LIN Bi-shan;GAO Yong-jian;LIU Yong;LIANG Si-yun;OU Shu-yun(Guangdong Medi-World Pharmaceutical Co.,Ltd.,Sinopharm Group,Foshan 528305,China)

机构地区：[1]国药集团广东环球制药有限公司,广东佛山528305

出　　处：《中国现代中药》2022年第10期1982-1987,共6页Modern Chinese Medicine

摘　　要：目的:建立地骨皮药材、饮片和标准汤剂超高效液相色谱法(UPLC)特征图谱及有效成分的定量检测方法,探讨其相关性,为地骨皮配方颗粒的质量标准制定提供参考。方法:采用UPLC,应用WatersBEHC_(18)色谱柱(100 mm×2.1 mm,1.7μm),甲醇-0.15%三氟乙酸水溶液为流动相,梯度洗脱,检测波长280 nm,流速0.25 mL·min^(-1),柱温40℃。根据定量检测、出膏率、特征图谱的测定结果,对药材-饮片-标准汤剂进行相关性分析。结果:药材、饮片、标准汤剂特征图谱均与各自的对照图谱相似度良好,分别标识出5个共有峰,指认了2个色谱峰。18批地骨皮标准汤剂平均出膏率为12.89%,地骨皮乙素从饮片到标准汤剂的平均转移率为44.84%,未出现离散数据。结论:采用特征图谱与出膏率、定量检测指标相结合的模式,对药材、饮片和标准汤剂的质量控制及相关性进行研究,可为地骨皮配方颗粒及经典名方的研究提供参考。Objective:To establish ultra-performance liquid chromatography(UPLC)characteristic chromatogram and quantitative detection of raw herb,decoction pieces and standard decoction of Lycii Cortex and evaluate the correlation,serving as a reference for the quality standard of Lycii Cortex formula granules.Methods:UPLC analysis was performed with the Waters BEH C_(18) column(100 mm×2.1 mm,1.7µm)and methanol-0.15%trifluoroacetic acid solution as the gradient mobile phase,and 280 nm was maintained as the detection wavelength.The flow rate was 0.25 mL·min^(−1) and the column temperature was 40℃.The correlation of raw herb,decoction pieces and standard decoction was analyzed based on the results of quantitative detection,extractum rate and characteristic chromatogram.Results:The characteristic chromatogram of raw herb,decoction pieces and standard decoction shared high similarity with the respective control chromatogram.Five common peaks were confirmed and two chromatographic peaks were identified.The average extractum rate of the 18 batches of standard decoction of Lycii Cortex was 12.89%,and the average transfer rate of kukoamine B from decoction pieces to standard decoction was 44.84%with no discrete data observed.Conclusion:The quality control and correlation between raw herbs,decoction pieces and standard decoction were investigated using the pattern of characteristic chromatogram combined with extractum rate and quantitative detection indicators,which can provide a reference for the research concerning Lycii Cortex formula granules and classic prescriptions.

关 键 词：地骨皮 标准汤剂 超高效液相色谱法 特征图谱 定量检测 相关性 

分 类 号：R286[医药卫生—中药学]