IL-1β诱导骨关节炎细胞模型中FSTL1蛋白的表达及意义  

Expression and significance of FSTL1 protein in IL-1β-induced osteoarthritis cell model

作　　者：敖沸[1] 杨德猛[2] 陶凌凤[3] 胡华平 贾芝和[1] 何敏[1] 杨慧文[1] AO Fei;YANG Demeng;TAO Lingfeng(Osteoarthrosurgery of Ping Xiang People's Hospital,Ping xiang,Jiang Xi 337000;Department of Orthopaedic Trauma of Ping Xiang People's Hospital,Ping xiang,Jiang Xi 337000;Nursing department of Ping Xiang People's Hospital,Ping xiang,Jiang Xi 337000)

机构地区：[1]江西省萍乡市人民医院骨关节外科,萍乡337000 [2]江西省萍乡市人民医院创伤骨科,萍乡337000 [3]江西省萍乡市人民医院护理部,萍乡337000

出　　处：《江西医药》2022年第9期1019-1022,共4页Jiangxi Medical Journal

基　　金：江西省卫生健康委科技计划,编号20202063。

摘　　要：目的探究卵泡抑素样蛋白1(FSTL1)在人关节软骨细胞内的表达情况及意义。方法采用IL-1β刺激人关节软骨细胞24 h建立细胞模型。免疫荧光技术检测FSTL1在正常人软骨细胞内的表达情况;Western Blot对IL-1β诱导的人关节软骨细胞FSTL1表达水平进行检测。结果免疫荧光结果显示,在正常人软骨细胞内FSTL1蛋白表达广泛且主要分布在细胞质;Western Blot结果显示,IL-1β诱导的人软骨细胞中FSTL1蛋白表达与正常组相比显著减少(P<0.01)。结论IL-1β诱导显著抑制人关节软骨细胞FSTL1蛋白表达,FSTL1可能是骨关节炎治疗的关键靶点。Objective To explore the expression and significance of follistatin-like protein 1(FSTL1)in human articular chondrocytes.Methods For establishing the cell model,Human articular chondrocytes were cultivated with IL-1βfor 24 hours.To analyze the expression of FSTL1 in Human articular chondrocytes,immunofluorescence was used.And to analyze the expression of FSTL1 in human articular chondrocytes induced by IL-1βwas detected,Western Blot was used.Results Immunofluorescence showed that FSTL1 was widely expressed in normal human chondrocytes and mainly distributed in the cytoplasm.Comparing the two groups,Western Blot showed that the expression of FSTL1 protein in human chondrocytes induced by IL-1βwas significantly decreased(P<0.01).Conclusion After the cell was cultivated with IL-1β,we study that the expression of FSTL1 protein in human articular chondrocytes was significantly reduced.In summary,these results show that FSTL1 may be a key target in the treatment of osteoarthritis.

关 键 词：骨关节炎 卵泡抑素样蛋白1 人关节软骨细胞 

分 类 号：R684.3[医药卫生—骨科学]