超声造影评估铁皮石斛对肝纤维化大鼠肾损伤时微循环灌注的影响  

Evaluation of the effect of Dendrobium officinale on microcirculation perfusion in renal injury of rats with hepatic fibrosis by contrast-enhanced ultrasound

作　　者：李红俊 王静 范钦 刘蜻蜻 陈海燕 洪文娟 余卓锐 成家茂 LI Hongjun;WANG Jing;FAN Qin;LIU Qingqing;CHEN Haiyan;HONG Wenjuan;YU Zhuorui;CHENG Jiamao(Department of Ultrasound,the First Affiliated Hospital of Dali University,Yunnan 671000,China)

机构地区：[1]大理大学第一附属医院超声科,671000 [2]大理大学基础医学院解剖学教研室 [3]大理大学第一附属医院肾内科,671000 [4]弥勒市中医医院放射科

出　　处：《临床超声医学杂志》2022年第11期805-810,共6页Journal of Clinical Ultrasound in Medicine

基　　金：2022年云南省教育厅科学研究基金项目(2022Y809);2022年度云南省教育厅科学研究基金面上项目(2022J0695);2021年云南省地方本科高校基础研究联合专项资金面上项目(202101BA070001-101);2021年云南省教育厅科学研究基金项目(2021J0382);2021年大理市科技计划项目(2021KBG009)。

摘　　要：目的 应用超声造影(CEUS)评估铁皮石斛对肝纤维化大鼠肾损伤时微循环灌注的影响。方法 将24只SD雄性大鼠随机分成正常组、模型组和铁皮石斛干预组,每组各8只,除正常组以外,其余两组均经四氯化碳诱导制备肝纤维化动物模型,其中铁皮石斛干预组以高剂量铁皮石斛鲜榨汁每日灌胃(7.5 g/kg),连续灌胃4周。应用常规超声观察大鼠肾脏形态并检测其长径、前后径、肾皮质厚度,CEUS检测肾皮质血流灌注参数即达峰时间(TTP)、峰值强度(PSI)、曲线上升斜率(A)和曲线下面积(AUC);收集大鼠处死前1 d的24 h尿液和处死后腹主动脉血液,应用全自动生化检测仪测定尿、血肌酐(Cr)和血尿素氮(BUN),并计算内生肌酐清除率(Ccr);比较各组上述参数的差异。取肝、肾组织进行HE及Masson染色观察病理学变化。结果 各组大鼠肾脏长径、前后径和肾皮质厚度比较差异均无统计学意义。CEUS检查显示,与正常组比较,模型组和铁皮石斛干预组大鼠肾皮质TTP延长,PSI和A均降低,AUC增加,差异均有统计学意义(均P<0.05);与模型组比较,铁皮石斛干预组大鼠肾皮质TTP缩短,PSI和A均增高,AUC减小,差异均有统计学意义(均P<0.05)。与正常组比较,模型组和铁皮石斛干预组大鼠血Cr、BUN水平升高,Ccr水平下降,差异均有统计学意义(均P<0.05);与模型组比较,铁皮石斛干预组大鼠血Cr、BUN水平下降,Ccr水平上升,差异均有统计学意义(均P<0.05)。光镜下,大鼠肝组织HE及Masson染色结果显示,模型组肝索排列紊乱,肝细胞内可见大量脂肪空泡,汇管区明显扩展,并见炎性细胞浸润、大量纤维间隔和假小叶形成;铁皮石斛干预组较模型组肝纤维化有不同程度改善,汇管区炎性细胞浸润有所减轻,纤维间隔和假小叶分布减少。肾组织HE染色结果显示,各组大鼠肾小球、肾小管和间质区均未见明显异常病理改变。结论 应用CEUS可实时、定量�Objective To evaluate the effect of Dendrobium officinale on microcirculation perfusion of renal injury of rats with hepatic fibrosis by contrast-enhanced ultrasound(CEUS).Methods Twenty-four male SD rats were randomly divided into normal group,model group and Dendrobium officinale group,with 8 rats in each group.Except normal group,rats of the rest two groups were induced by carbon tetrachloride to prepare hepatic fibrosis animal models.Rats of Dendrobium officinale group were treated with high-dose fresh extract of Dendrobium officinale(7.5 g/kg)by gavage for 4 weeks.Conventional ultrasound and CEUS were used to observe and compare the changes in long diameter,anteroposterior diameter and cortical thickness of kidney in each group,as well as the renal cortical blood flow perfusion,including time to peak(TTP),peak intensity(PSI),ascending slope(A)and area under the curve(AUC).The 24 h urine before execution 1 d and abdominal aorta bleed after execution were collected.Urine,blood creatinine(Cr)and blood urea nitrogen(BUN)were measured by automatic biochemical detector,and endogenous creatinine clearance(Ccr)was calculated.The above parameters among the groups were compared.Liver and kidney tissues were taken for HE and Masson staining to observe the pathological changes.Results The long diameter,anteroposterior diameter and cortical thickness of kidney in each group had no statistically difference.Compared with the normal group,CEUS showed that TTP in renal cortex was prolonged,PSI and A were decreased,and AUC was increased in the model group and Dendrobium officinale group(all P<0.05).Compared with the model group,TTP in renal cortex was shortened,PSI and A were increased,and AUC was decreased in the Dendrobium officinale group,the differences were statistically significant(all P<0.05).Compared with the normal group,the blood Cr and BUN levels were increased,Ccr was decreased in the model group and Dendrobium officinale group,the differences were statistically significant(all P<0.05).Compared with the model grou

关 键 词：超声检查 造影剂 时间-强度曲线 铁皮石斛 肾脏血流灌注 肝纤维化 大鼠 

分 类 号：R445.1[医药卫生—影像医学与核医学] R575.2[医药卫生—诊断学]