Integrated network analyses identify MYB4R1 neofunctionalization in the UV-B adaptation of Tartary buckwheat  

作　　者：Moyang Liu Wenjun Sun Zhaotang Ma Chaocheng Guo Jiahao Chen Qi Wu Xiyin Wang Hui Chen 

机构地区：[1]College of Life Science,Sichuan Agricultural University,Ya’an 625014,China [2]Joint Center for Single Cell Biology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [3]Shanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [4]School of Life Science,North China University of Science and Technology,Tangshan 063210,China [5]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Key Laboratory of Major Crop Diseases and Rice Research Institute,Sichuan Agricultural University,Chengdu 611130,China

出　　处：《Plant Communications》2022年第6期267-281,共15页植物通讯（英文）

基　　金：sponsored by the National Key R&D Program of China(2021YFD1200105);the National Natural Science Foundation of China(3210150112);the Sichuan Province Science and Technology Support Program(2021YFH0086);the Shanghai Sailing Program(20YF1422000);the Startup Fund for Youngman Research at Shanghai Jiao Tong University(20X100040052).

摘　　要：A hallmark of adaptive evolution is innovation in gene function,which is associated with the development of distinct roles for genes during plant evolution;however,assessing functional innovation over long periods of time is not trivial.Tartary buckwheat(Fagopyrum tataricum)originated in the Himalayan region and has been exposed to intense UV-B radiation for a long time,making it an ideal species for studying novel UV-B response mechanisms in plants.Here,we developed a workflow to obtain a co-functional network of UV-B responses using data from more than 10,000 samples in more than 80 projects with multi-species and multiomics data.Dissecting the entire network revealed that flavonoid biosynthesis was most significantly related to the UV-B response.Importantly,we found that the regulatory factor MYB4R1,which resides at the core of the network,has undergone neofunctionalization.In vitro and in vivo experiments demonstrated that MYB4R1 regulates flavonoid and anthocyanin accumulation in response to UV-B in buckwheat by binding to L-box motifs in the FtCHS,FtFLS,and FtUFGT promoters.We used deep learning to develop a visual discrimination model of buckwheat flavonoid content based on natural populations exposed to global UV-B radiation.Our study highlights the critical role of gene neofunctionalization in UV-B adaptation.

关 键 词：NEOFUNCTIONALIZATION deep learning MYB UV-B radiation Tartary buckwheat 

分 类 号：Q94[生物学—植物学]