磷酸化蛋白质组学研究中磷酸化肽段富集与分离方法研究进展  

Advances in the methods of phosphopeptide enrichment and separation in phosphoproteomic research

作　　者：李佳然 陈秀兰[1,2] 杨福全 LI Jiaran;CHEN Xiulan;YANG Fuquan(Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院生物物理研究所,北京100101 [2]中国科学院大学,北京100049

出　　处：《生物工程学报》2022年第10期3648-3658,共11页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2018YFA0507801)。

摘　　要：对磷酸化蛋白质组(phosphoproteome)进行系统深入的研究依赖于高重复性和特异性的磷酸化肽段富集与分离方法。目前发展了多种不同原理的磷酸化肽段富集方法,它们往往具有不同的选择性和特异性,因此,根据不同的研究目的选择最适合的富集方法显得尤为重要。本文综述了基于亲和色谱法(affinity chromatography)、免疫沉淀法(immunoprecipitation)、化学衍生法(chemical derivatization)、色谱法(chromatography)和其他新发展方法的磷酸化肽段富集方法,详细介绍了各自的优缺点及相关的优化与改进策略。此外,还简单介绍了磷酸化肽段富集与预分方法的不同组合的研究进展。The systematic and in-depth study of phosphoproteome rely on highly reproducible and specific phosphopeptide enrichment methods.At present,a variety of enrichment methods have been developed based on different principles,and these methods often display different selectivity and specificity.It is therefore very important to select the most suitable enrichment method according to different research purposes.This review summarized the phosphopeptide enrichment based on affinity chromatography,immunoprecipitation,chemical derivatization,chromatography and other newly developed methods.The advantages and disadvantages of these methods,as well as the related optimization and improvement strategies,were discussed in detail.In addition,we also briefly summarized the progress of the combination of phosphopeptide enrichment and fractionation methods developed in recent years.

关 键 词：磷酸化蛋白质组学 磷酸化肽段 磷酸化肽段富集 磷酸化肽段预分 

分 类 号：Q503[生物学—生物化学]