Antiviral effects of natural small molecules on aquatic rhabdovirus by interfering with early viral replication  

作　　者：Yan Zhou Tian-Xiu Qiu Yang Hu Lei Liu Jiong Chen 

机构地区：[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Ningbo University,Ningbo,Zhejiang 315211,China [2]Laboratory of Biochemistry and Molecular Biology,School of Marine Sciences,Meishan Campus,Ningbo University,Ningbo,Zhejiang 315832,China [3]Key Laboratory of Applied Marine Biotechnology of Ministry of Education,Meishan Campus,Ningbo University,Ningbo,Zhejiang 315832,China

出　　处：《Zoological Research》2022年第6期966-976,共11页动物学研究（英文）

基　　金：supported by the National Natural Science Foundation of China(31902410);Natural Science Foundation of Zhejiang Province(LQ22C190002);Natural Science Foundation of Ningbo City(2021J117);Program of Science and Technology Department of Ningbo City(2021S058);One Health Interdisciplinary Research Project of Ningbo University(HZ202201);Zhejiang Xinmiao Talents Programs(2022R405B066)。

摘　　要：Spring viremia of carp virus(SVCV)is globally widespread and poses a serious threat to aquatic ecology and aquaculture due to its broad host range.To develop effective agents to control SVCV infection,we selected 16 naturally active small molecules to assess their anti-SVCV activity.Notably,dihydroartemisinin(DHA)(100μmol/L)and(S,S)-(+)-tetrandrine(TET)(16μmol/L)exhibited high antiviral effects in epithelioma papulosum cyprinid(EPC)cells,with inhibitory rates of 70.11%and 73.54%,respectively.The possible antiviral mechanisms were determined as follows:1.Preincubation with DHA and TET decreased viral particle infectivity in fish cells,suggesting that horizontal transmission of SVCV in the aquatic environment was disrupted;2.Although neither had an effect on viral adhesion,TET(but not DHA)interfered with SVCV entry into host cells(>80%),suggesting that TET may have an antiviral function in early viral replication.For in vivo study,both agents enhanced the survival rate of SVCV-infected zebrafish by 53.3%,significantly decreased viral load,and modulated the expression of antiviralrelated genes,indicating that DHA and TET may stimulate the host innate immune response to prevent viral infection.Overall,our findings indicated that DHA and TET had positive effects on suppressing SVCV infection by affecting early-stage viral replication,thus holding great potential as immunostimulants to reduce the risk of aquatic rhabdovirus disease outbreaks.

关 键 词：Spring viremia of carp virus Natural small molecules Antiviral effects Virus internalization Danio rerio 

分 类 号：S941.41[农业科学—水产养殖]