斧翅沙芥种子浸提液成分分析及其对斧翅沙芥和白菜种子萌发的影响  

Analysis on the Components of the Extract from Pugionium dolabratum Seed and Its Effect on the Seed Germination of the Chinese Cabbage Pugionium dolabratum

作　　者：常小瑶 蒋玉婷[1] 黄修梅[1] 郝丽珍[1] 杨忠仁 张凤兰[1] CHANG Xiaoyao;JIANG Yuting;HUANG Xiumei;HAO Lizhen;YANG Zhongren;ZHANG Fenglan(Key Laboratory of Germplasm Resources and Germplasm Innovation of Wild Endemic Vegetables in Inner Mongolia,College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010011,China;Key Laboratory of Big Data Research and Application of Agriculture and Animal Husbandry of Inner Mongolia Autonomous Region,Hohhot 010011,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院/内蒙古野生特有蔬菜种质资源与种质创新重点实验室,呼和浩特010011 [2]内蒙古自治区农牧业大数据研究与应用重点实验室,呼和浩特010011

出　　处：《种子》2022年第10期43-49,57,共8页Seed

基　　金：国家自然科学(31760570);国家重点研发计划重点专项(2021 YFD 200200);内蒙古自治区应用技术研究与开发项目(2019 GC 237);内蒙古自治区科技成果转化专项资金项目(2021 CG 0023);内蒙古自治区科技计划项目(2021 GG 0084)。

摘　　要：采用有机溶剂萃取的方法对斧翅沙芥种子内源物质进行分离和生物测定,同时结合气相色谱-质谱(GC-MS)联用仪分析其内源抑制物质的成分和含量。结果表明,斧翅沙芥休眠及非休眠种子浸提液对白菜和非休眠斧翅沙芥种子的发芽率存在抑制作用,且休眠种子浸提液的抑制大于非休眠种子;对斧翅沙芥种子浸提液初步分离得到各有机相,各有机相对斧翅沙芥种子萌发的抑制作用为乙酸乙酯相=甲醇相>乙醚相>水相>石油醚相,表明这些抑制物质主要集中于乙酸乙酯相与甲醇相中;对有机相分离鉴定发现,斧翅沙芥种子中存在的内源抑制物主要以酯类、苯类、有机酸类、酚类、醚类和醇类等。研究认为,这些抑制物质的存在是斧翅沙芥种子休眠的原因之一。The organic solvent extraction method was used to isolate and biodetermine the endogenous substances in the seeds of Pugionium dolabratum,and the composition and content of the endogenous inhibitory substances were analyzed by gas chromatography mass spectrometry(GC-MS).The results showed that the germination percentage of Chinese cabbage and non-dormancy seeds was inhibited by the extracts of dormancy seeds and non-dormancy seeds of Pugionium dolabratum,and the inhibition of the extracts of dormancy seeds was greater than that of non-dormancy seeds.Each organic phase was obtained from the seed extract of Pugionium dolabratum,and the inhibitory effect of each organic phase on seed germination was ethyl acetate phase=methanol phase>ethyl ether phase>aqueous phase>petroleum ether phase,indicating that these inhibitory substances mainly concentrated in ethyl acetate phase and methanol phase.The results of organic phase separation and identification showed that the endogenous inhibitors in the seeds were mainly esters,benzenes,organic acids,phenols,ethers and alcohols.It was concluded that the existence of these inhibitory substances was one of the reasons for seed dormancy.

关 键 词：斧翅沙芥 种子 内源物质 气相色谱-质谱联用仪 

分 类 号：S649[农业科学—蔬菜学]