^(60)Co辐射诱导水稻短穗小粒突变体sp18的表型鉴定与基因定位  

Phenotypic Identification and Gene Mapping of the Short-panicle and Small-grain Mutant sp18 in Rice through ^(60)Co Radiation-induced

作　　者：陈能刚 鄢小青 李欢 陈锋[1] CHEN Nenggang;YAN Xiaoqing;Li Huan;CHEN Feng(Institute of Crop Germplasm Resources,GuizhouAcademy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州省农业科学院农作物品种资源研究所,贵阳550006

出　　处：《种子》2022年第10期90-94,共5页Seed

基　　金：贵州省自然科学基金项目“水稻粒宽新基因GS114的基因克隆与功能研究”(黔科合基础-ZK[2022]一般243);贵州省农业科学院院引导项目“贵州作物种质资源信息管理平台建设及地方特色作物的收集保护”(黔农科院引导[2018]05号);贵州省科技支撑计划项目“水稻同质渗粳恢复系的创制与利用研究”(黔科合支撑[2018]2300)。

摘　　要：利用^(60)Co辐射诱变贵州地方粳稻品种桥港珍珠米获得短穗小粒突变体sp18,鉴定其表型特征,并进行遗传与分子标记连锁分析,全基因组重组序列分析及突变基因分析。结果表明,与野生型相比,突变体sp18的株高增加,有效穗没有显著变化,而结实率、穗长、穗粒数和千粒重较野生型显著减少。利用水稻SSR标记进行初步定位,发现第11号染色体上的分子标记RM 167和RM 27150与目的基因连锁,物理距离分别为19.1 cM和41.5 cM处。突变体和野生型进行全基因组重测序分析,发现sp18在第11号染色体上缺失322720 bp(7189868~7512630 bp之间)。在缺失区域内包括Os11g0235200、Os11g0235700、Os11g0235750、Os11g0236000、Os11g0236100、Os11g0236200、Os11g0237900、Os11g0238000、Os11g0238400、Os11g0238700、Os11g0239000、Os11g0239200、Os11g0239400、Os11g0239500、Os11g0240600和Os11g0240900。其中Os11g0235200编码肽转运蛋白,为已克隆的短穗基因sp1。Os11g0236000编码磷酸甘油二酯磷酸二酯酶家族蛋白,参与生长素信号传导。Os11g0239500和Os11g0240600均编码赤霉素受体GID 1 L 2,参与赤霉素的调控。因此,短穗小粒突变基因sp18为sp1的新等位基因,然而sp18突变体呈现出与sp1既相似而又不同的突变表型特征,推测可能是sp18基因组大片段缺失,包括多个与植物激素相关的基因的缺失而导致的。A short-spike small-grain mutant sp18 was obtained by ^(60)Co radiation irradiated the seeds of the Qiao-gang pearl rice,a japonica rice germplasm resource in Guihzou,of which phenotypic traits were identificated,genetic and molecular marker chain,the whole genome recombinat sequence and mutant gene were analysed.The results showed that compared to the wild-type,the plant-height increased and the effective panicle did not change significantly,but the seed setting rate,panicle length,grain number per-panicle and 1000-grain weight decreased significantly in sp18.By SSR molecular marker analysis,it was found that the markers RM 167 and RM 27150 were linked to the target gene with physical distances of 19.1 cM and 41.5 cM on chromosome 11,respectively.Furthermore,using the genome-wide resequencing to analyze the sp18 and the wild-types,it revealed that sp18 was 322720 bp(between 7189868 bp and 7512630 bp)deleted on chromosome 11.The deletion region included Os11g0235200,Os11g0235700,Os11g0235750,Os11g0236000,Os11g0236100,Os11g0236200,Os11g0237900,Os11g0238000,Os11g0238400,Os11g0238700,Os11g0239000,Os11g0239200,Os11g0239400,Os11g0239500,Os11g0240600 and Os11g0240900,of which Os11g0235200,encoding peptide transporter,was a cloned short-spike gene SP1.Os11g0236000 encoded phosphodiester phosphodiesterase family proteins,which participated in auxin signal transduction.And including both Os11g0239500 and Os11g0240600 encoded gibberellin receptor GID1L 2,which participate in the regulation of gibberellin.Therefore,the sp18 was a new allele of sp1.However,it was speculated that the sp18 mutant eventually exhibitted a similar but different mutant phenotype from sp1 due to the large fragment deletion,including multiple genes related to plant hormones.

关 键 词：水稻 短穗 小粒 变异分析 

分 类 号：S511[农业科学—作物学]