文昌鸡中脂联素受体CDH13基因的表达  

Expression pattern of adiponectin receptor gene CDH13 in Wenchang Chickens

作　　者：王颖[1] 吴红芬 张颖[1] 汪刘浩 那威 吴科榜[1] WANG Ying;WU Hongfen;ZHANG Ying;WANG Liuhao;NA Wei;WU Kebang(college of animal science and technology of hainanu university)

机构地区：[1]海南大学动物科技学院,海口570228

出　　处：《热带生物学报》2022年第6期558-561,共4页Journal of Tropical Biology

基　　金：海南省基础与应用基础研究计划高层次人才项目(2019RC101)。

摘　　要：以文昌鸡为实验材料,利用RT-qPCR的方法检测在文昌鸡不同组织及腹部脂肪组织生长发育过程中CDH13的表达情况,为鸡CDH13基因的功能研究奠定基础。研究结果显示,CDH13基因在心脏、回肠、脂肪组织、小脑及肌胃中表达量较高,脂联素表达情况与之相似;CDH13基因在文昌鸡腹部脂肪组织生长发育过程中均稳定高表达,并育肥4周龄时,表达量达到最高。Wenchang Chicken is a local excellent broiler breed in Hainan,which has the advantages of strong adaptability,heat resistance and good meat quality.At present,there is a problem with high abdominal fat rate in Wenchang Chickens.Excessive fat deposition is undesirable since it decreases feed efficiency,and affects human health.Adiponectin,which is secreted by mature adipose cells,plays an important role in lipid metabolism.CDH13 is the third type of receptor for adiponectin,and the function of chicken CDH13 has not yet been reported.Therefore,the expression pattern of CDH13 in different tissues and during the growth and development of the abdominal adipose tissue of Wenchang Chickens was determined by using the RT-qPCR.The results showed that the gene CDH13 had higher mRNA abundance in the heart,ileum,adipose tissue,cerebellum and gizzard,and that adiponectin had similar trend of transcriptional levels to CDH13.The gene CDH13 was stably and highly expressed during the growth and development of abdominal adipose tissue,and its expression level was the highest at the age of the fourth week in the fattening period.

关 键 词：文昌鸡 CDH13 脂联素受体 表达规律 

分 类 号：S831.2[农业科学—畜牧学] Q786[农业科学—畜牧兽医]