Regulatory Effects of Zuogui Pill on Apoptosis of Follicles in Rats Injured by 60Co-γRays Based on PI3K/Akt/m TOR Signaling Pathway  

作　　者：Fenqin ZHAO Mingxia AN Xiaonan DING Jieying LIU Yan ZHAO Zhihui XIE Shuping LI 

机构地区：[1]Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China [2]Gansu Provincial People's Hospital,Lanzhou 730000,China

出　　处：《Medicinal Plant》2022年第5期45-50,58,共7页药用植物：英文版

基　　金：Supported by National Natural Science Foundation of China(81760806);Project of Traditional Chinese Medicine Administration of Gansu Province(GZK-2019-28);Innovation Ability Improvement Project of Higher Education Institutions of Gansu Province(2019B-103)。

摘　　要：[Objectives]To explore the protective effects of Zuogui Pill on ^(60)Co-γ-ray-induced premature aging of rats based on phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR)signaling pathway.[Methods]Sixty sexually mature female SD rats were irradiated with ^(60)Co-γ-ray(6.0 Gy,LD 40)for 24 h at one time.These rats were randomly divided into model group,Progynova group[0.18(g·kg)/d],Progynova[0.09(g·kg)/d]+Zuogui Pill high dose[23.625(g·kg)/d)]group,Zuogui Pill high dose[23.625(g·kg)/d)]group,Zuogui Pill medium dose[9.45(g·kg)/d)]group and Zuogui Pill low dose[4.725(g·kg)/d]group.The administration(once a day)lasted 21 d.The rat serum[follicle-stimulating hormone(FSH),luteinizing hormone(LH)and estradiol(E_(2))]were detected by Enzyme-linked immunosorbent assay(ELISA).The morphological changes of ovary were observed by hematoxylin-eosin(HE)staining.The apoptosis rate of granulosa cells was detected by terminal deoxynucleotidyl transferase(TdT)-mediated dUTP nick-end labeling(TUNEL).The protein expression of phosphorylated(p)-PI3K,p-Akt,p-mTOR,B-cell lymphoma-2(Bcl-2),and Bcl-2-associated X protein(Bax)in ovarian tissues were detected by Western blot.[Results]Compared with the normal group,the model group showed significant increase in the serum FSH(P<0.01),significant decrease in serum E_(2)(P<0.05),and decrease in the number of early follicles and luteum in the ovary(P<0.01).Besides,the apoptosis rate of granulosa cells increased significantly(P<0.01);the expression of p-PI3K,p-Akt,p-mTOR and Bcl-2 in ovarian tissue decreased significantly,while the expression of Bax increased significantly(P<0.01).Compared with the model group,the number of early follicles in the ovary increased and the apoptosis rate of granulosa cells decreased after intervention in each administration group.In addition,the protein expressions of p-PI3K,p-Akt,p-mTOR and Bcl-2 increased,while the expression of Bax decreased,especially in Progynova+Zuogui Pill high dose group,the differences were sta

关 键 词：Radiation injury Premature ovarian failure(POF) Zuogui Pill Terminal deoxynucleotidyl transferase(TdT)-mediated dUTP nick-end labeling(TUNEL) Phosphatidylinositol-3-kinases/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR)signaling pathway B-cell lymphoma-2 Bcl-2-associated X protein 

分 类 号：R285.5[医药卫生—中药学]