大麻PEBP基因家族鉴定及生物信息学分析  

Identification and Bioinformatics of PEBP Family in Hemp

作　　者：陈晗 徐洪国[1,2] 王志刚 徐伟慧[1,2] 葛宵启[1] 祁宏英[1,2] CHEN Han;XU Hongguo;WANG Zhigang;XU Weihui;GE Xiaoqi;QI Hongying(College of Life Sciences,Agriculture and Forestry,Qiqihar University,Qiqihar,Heilongjiang 161006,China;Engineering Research Center of Hemp and Hemp Products in Cold Regions,Ministry of Education,Qiqihar,Heilongjiang 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]寒区麻及制品教育部工程研究中心,黑龙江齐齐哈尔161006

出　　处：《福建农业学报》2022年第8期1016-1024,共9页Fujian Journal of Agricultural Sciences

基　　金：黑龙江省省属本科高校基本科研业务费工业大麻专项(135409704)。

摘　　要：【目的】对大麻中磷脂酰乙醇胺结合蛋白(phosphatidyl ethanolamine binding protein,PEBP)进行鉴定和生物信息学分析,为PEBP基因家族调控大麻的生长发育及开花奠定基础。【方法】以拟南芥PEBP家族基因作为参考序列,利用NCBI、MEME、TBtools等生物信息学工具对已有的大麻基因组数据进行筛选并鉴定大麻PEBP基因。【结果】从大麻基因组中共筛选出12个PEBP基因,分为FT-LIKE、TFL-LIKE、MET-LIKE三个亚家族,均为亲水性蛋白,分布于细胞质与细胞核中;motif1-motif5为大麻PEBP基因的特征基序,定位在7条染色体上。大麻PEBP基因含有2个外显子及2个以上内含子,其启动子主要含有光反应顺式调节元件、各类激素顺式调控元件,编码蛋白主要由无规则卷曲构成。【结论】共鉴定出12个CsPEBP基因家族成员,通过motif分析、多物种构建进化树,确定大麻PEBP基因具有高度保守性,并预测和分析其潜在的分子功能。【Objective】The phosphatidyl ethanolamine binding protein(PEBP)in Cannabis sativa L.(hemp)was identified and bioinformatics of the gene family analyzed.【Method】Using the PEBP family of arabidopsis as reference,the existing genome data on C.sativa were collected by NCBI,MEME,and TBtools.【Result】Twelve PEBPs were identified from the database.They were divided into 3 subfamilies as FT-like,TFL-like,and MET-like.These hydrophilic proteins were distributed in cytoplasm and nucleus with the characteristic motif1-motif5 of cannabis PEBP that located on 7 chromosomes.The genes contained 2 exons and more than 2 introns with a promoter containing mainly light-responsive cis-regulatory elements and various hormone cis-regulatory elements.The encoded protein composed basically of random coils.【Result】Twelve genes in the PEBP family of C.sativa were identified with their potential molecular functions analyzed.

关 键 词：工业大麻 PEBP基因 功能鉴定 生物信息学分析 

分 类 号：S668.4[农业科学—果树学]