番茄白粉病室内苗期抗病性鉴定方法研究及种质资源抗病性评价  

Indoor seedling identification method research of tomato resistance topowdery mildew and disease resistance evaluation of its germplasm resources

作　　者：李小红 赵雯 周鹏泽 周雪荣 马雪静 杨雯雯 田飞燕 王晓敏[1,3,4,5] LI Xiaohong;ZHAO Wen;ZHOU Pengze;ZHOU Xuerong;MA Xuejing;YANG Wenwen;TIAN Feiyan;WANG Xiaomin(School of Agriculture,Ningxia University,Yinchuan 750021,Ningxia,China;Federal Science and Engineering Organization of Australia,Canberra 2600,Australia;Ningxia Modern Facility Horticulture Engineering Technology Research Center,Yinchuan 750021,Ningxia,China;Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan 750021,Ningxia,China;Ningxia Facility Horticulture(Ningxia University)Technology Innovation Center,Yinchuan 750021,Ningxia,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]澳大利亚联邦科工组织,澳大利亚堪培拉2600 [3]宁夏现代设施园艺工程技术研究中心,银川750021 [4]宁夏优势特色作物现代分子育种重点实验室,银川750021 [5]宁夏设施园艺(宁夏大学)技术创新中心,银川750021

出　　处：《中国瓜菜》2022年第11期80-85,共6页China Cucurbits And Vegetables

基　　金：宁夏回族自治区区级大学生创新创业训练计划项目(Q2020107490059);国家自然科学基金项目(31860561);宁夏大学园艺国家一流本科专业建设项目(2020);宁夏回族自治区农业特色优势产业育种专项“瓜菜种质资源创新与新品种选育(2020—2024)”(NXNYYZ20200101)。

摘　　要：为了筛选番茄白粉病室内苗期最适的抗病性鉴定方法,并利用该方法对30份番茄种质进行抗病性评价,以常温保存和新鲜孢子作对照,通过设置不同的保存方法(低温保存法、甘油保存法和二甲基亚砜保存法)比较孢子萌发率、病情指数,筛选最佳的保存条件;采用四因素三水平正交试验设计,对比发病率和病情指数。结果表明,除新鲜孢子外,4℃条件下保存30、60、90 d后孢子萌发率和病情指数分别为44.20%、22.50;39.60%、22.00;27.50%、16.30,在相应保存时间下均高于其他方法。(处理5)4叶期,孢子浓度为1×10^(6)个·mL^(-1),刷叶法,保湿24 h条件下的发病率和病情指数分别是76.45%、46.06,高于其他处理;筛选出抗病材料16份。最终得出4℃是最佳的白粉菌保存条件;4叶期,孢子浓度为1×10^(6)个·mL^(-1),刷叶法,保湿24 h是最佳的番茄白粉病室内苗期抗病性鉴定条件,最抗病的番茄材料是62850-1。The identification method of indoor disease resistance at seedling stage of powdery mildew in tomato was screened,and by this method,the resistance of 30 tomato germplasms were evaluated.In this study,room temperature preservation and fresh spores were used as control,and different preservation methods were set:low temperature preservation,glycerol preservation and dimethy sulfoxide preservation.The spore germination rate and disease index were compared to screen the best preservation conditions.Four-factor and three-level orthogonal design was used to compare the incidence and disease index.The results showed that in addition to CK2,after preservation(30,60,90 d)at 4℃,the spore germination rate and disease index were 44.20%and 22.50,39.60%and 22.00,27.50%and 16.30,which were higher than other methods.The incidence rate and disease index of treatment 5 that was in four-leaf stage,the spore concentration was 1×10^(6) spores·mL^(-1),using brushing method and under 24 h moisturizing condition,were 76.45%and 46.06 respectively,which were higher than other treatments.16 resistant materials were screened.The results showed that 4℃was the best preservation condition for powdery mildew;Four-leaf stages,spore concentration is 1×10^(6) spores·mL^(−1),using leaf brushing method,moisturizing 24 h were the best condition for indoor seedling resistance identification method of tomato powdery mildew,the most resistant tomato material was 62850-1.

关 键 词：番茄 白粉病 抗性鉴定 种质资源 

分 类 号：S641.2[农业科学—蔬菜学]