针刺“三阴交”对微波辐射诱导的少弱精子症小鼠生精细胞的作用研究  

Effect of manual acupuncture at “Sanyinjiao” (SP6) on spermatogenic cells of mice with oligoasthenospermia induced by microwave radiation

作　　者：陈天帷 李海松[1] 毕焕洲 李志刚[3] 马文珠[3] 姜美娴[4] 于珊珊 叶雯佳 CHEN Tian-wei;LI Hai-song;BI Huan-zhou;LI Zhi-gang;MA Wen-zhu;JIANG Mei-xian;YU Shan-shan;YE Wen-jia(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Shenzhen Hospital of Beijing University of Chinese Medicine(Longgang),Shenzhen 518000,Guangdong Province;School of Acupuncuture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 10o029;The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110034)

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京中医药大学深圳医院(龙岗),广东深圳518000 [3]北京中医药大学针灸推拿学院,北京100029 [4]辽宁中医药大学附属第二医院,沈阳110034

出　　处：《针刺研究》2022年第10期891-895,共5页Acupuncture Research

基　　金：国家自然科学基金项目(No.81973845);深圳市龙岗区科技发展专项基金项目(No.LGKCYLWS2020066)。

摘　　要：目的:观察针刺“三阴交”对微波辐射诱导的少弱精子症小鼠精子活力及睾丸B细胞淋巴瘤/白血病2(Bcl-2)、Bcl-2相关X蛋白(Bax)和半胱氨酸蛋白酶-3(Caspase-3)的影响,探讨针刺改善少弱精子症的作用机制。方法:将BALB/C雄性小鼠随机分为空白组、模型组、针刺组,每组6只。采用频率为2450 MHz、功率密度为40 mW/cm^(2)的连续微波辐射1 h/d,共18 d,制备少弱精子症小鼠模型。针刺组造模同时每日手针针刺小鼠双侧“三阴交”各30 s,共18 d。采用计算机辅助精子分析法检测小鼠精子活力;HE染色法观察小鼠睾丸组织形态并计算Johnson评分;免疫荧光染色法检测小鼠睾丸Bcl-2、Bax和Caspase-3的阳性表达。结果:与空白组比较,模型组小鼠精液中前向运动(PR)精子、非前向运动(NP)精子百分比及总活率均降低(P<0.05),小鼠睾丸组织Johnson评分及Bcl-2平均荧光强度降低(P<0.05),Bax、Caspase-3平均荧光强度升高(P<0.05);与模型组比较,针刺组小鼠精液中PR精子百分比及总活率均升高(P<0.05),睾丸组织Johnson评分及Bcl-2平均荧光强度升高(P<0.05),Bax、Caspase-3平均荧光强度降低(P<0.05)。空白组小鼠睾丸的生精小管未见异常,可见各级生精细胞和精子,支持细胞排列整齐;模型组生精小管变薄,生精细胞和精子数量减少甚至消失,支持细胞部分缺失;针刺组上述情况较模型组有所改善。结论:针刺“三阴交”对微波辐射诱导的少弱精子症小鼠精子活力有保护作用,其机制可能与针刺降低睾丸生精微环境中的Bax和Caspase-3,升高Bcl-2有关。Objective To observe the effect of acupuncture at “Sanyinjiao”(SP6) on sperm motility, testicular B cell lymphoma/leukelia-2(Bcl-2), Bcl-2 associated X protein(Bax), and Caspase-3 in mice with oligoasthenospermia induced by microwave radiation, so as to explore its underlying mechanisms in improving oligoasthenospermia. Methods Male BALB/C mice were randomly divided into control, model and acupuncture groups(n=6 in each group). The oligoasthenospermia model was established by continuous microwave irradiation with frequency of 2 450 MHz and power density of 40 mW/cm^(2), 1 h daily for 18 days. At the same time, manual acupuncture was applied to the acupuncture group on bilateral “Sanyinjiao”(SP6) for 30 s, once daily for 18 days. Sperm motility including the percentages of progressive motility(PR), non-progressive motility(NP), and PR + NP sperms was detected by computer-assisted sperm analysis, H.E. staining was used to observe the testicular morphology and Johnson score was calculated, the expression levels of Bcl-2, Bax and Caspase-3 in testis were detected by immunofluorescence. Results Compared with the control group, the percentages of PR sperms, NP sperms, PR+NP sperms, Johnson score, and expression level of Bcl-2 were significantly decreased(P<0.05), while the expression levels of Bax and Caspase-3 were increased(P<0.05) in the model group. Compared with the model group, the percentages of PR sperms, PR+NP sperms, Johnson score, and expression level of Bcl-2 were significantly increased(P<0.05), while the expression levels of Bax and Caspase-3 were decreased(P<0.05) in the acupuncture group. Outcomes of H.E. staining showed that the seminiferous tubules became thinner, spermatogenic cells and sperm decreased or even disappeared, and the supporting cells were partially missing in the model group, which was relatively milder in the acupuncture group. Conclusion Manual acupuncture at SP6 can improve sperm motility in oligoasthenospermia mice induced by microwave radiation, which may be related to its

关 键 词：针刺 三阴交 微波辐射 少弱精子症 精子活力 凋亡 

分 类 号：R245.311[医药卫生—针灸推拿学]