黄芩苷对脂多糖诱导的人单核细胞THP-1的炎症反应的作用  

Effect of baicalin on lipopolysaccharide-stimulated inflammatory response in human monocytes THP-1

作　　者：王平[1,2] 杨军 王向东[1,2] 张罗[1,2,3] Wang Ping;Yang Jun;Wang Xiangdong;Zhang Luo(Department of Otorhinolaryngology Head and Neck Surgery and Allergy,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;Beijing Institute of Otorhinolaryngology,Beijing 100005,China;Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases,Chinese Academy of Medical Sciences,Beijing 100005,China)

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京100730 [2]北京市耳鼻咽喉科研究所,北京100005 [3]中国医学科学院慢性鼻病诊疗策略研究创新单元,北京100005

出　　处：《首都医科大学学报》2022年第6期893-898,共6页Journal of Capital Medical University

基　　金：教育部长江学者创新团队(IRT13082);中国医学科学院医学与健康科技创新工程项目(2019-I2M-5-022);北京市科技计划首都健康保障培育研究项目(Z181100001618002)。

摘　　要：目的探讨黄芩苷(baicalin)对脂多糖(lipopolysaccharide,LPS)诱导的人单核细胞THP-1的炎症反应的作用。方法利用LPS建立人单核细胞THP-1的炎症模型,实时荧光定量聚合酶链式反应(real-time quantitatFive polymerase chain reaction,RT-qPCR)方法检测白细胞介素-1β(interleukin-1β,IL-1β)、IL-6、IL-8、粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony-stimulating factor,GM-CSF)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的相对表达,确定最优的LPS刺激浓度。乳酸脱氢酶检测试剂盒检测黄芩苷的细胞毒性,优选其最佳使用浓度范围。对LPS诱导的人单核细胞THP-1炎症模型予以不同浓度的黄芩苷预处理,利用RT-qPCR法检测黄芩苷对LPS诱导的人单核细胞THP-1的IL-1β、IL-6、IL-8、GM-CSF和TNF-α的表达升高的影响。结果与对照组相比,1μg/mL的LPS处理人单核细胞THP-124 h后均可显著引起IL-1β、IL-6、IL-8、GM-CSF和TNF-α的表达升高(P<0.05),黄芩苷在浓度为10、30、100μmol/L时处理人单核细胞THP-124 h和48 h,无明显细胞毒性。30μmol/L和100μmol/L的黄芩苷可降低LPS诱导的人单核细胞THP-1的IL-1β、IL-6、IL-8、GM-CSF和TNF-α的表达升高。结论黄芩苷可显著抑制LPS诱导的人单核细胞THP-1的炎症反应。Objective To observe the effect of baicalin on the lipopolysaccharide(LPS)-stimulated inflammatory response in human monocytes THP-1.Methods LPS was used to induce the inflammatory response of human monocyte THP-1,and the relative expression levels of interleukin-1β(IL-1β),IL-6,IL-8,granulocyte-macrophage colony-stimulating factor(GM-CSF)and tumor necrosis factor-α(TNF-α)were detected by real-time quantitative polymerase chain reaction(RT-qPCR)method to optimize the stimulation concentration of LPS.The cytotoxic effects of baicalein were also detected using the lactate dehydrogenase assay kit to optimize the bacailin concentration.The LPS-induced inflammation model of human monocyte THP-1 was pretreated with baicalin at different concentrations,and the inhibition effect of baicalin on the increasing expression of IL-1β,IL-6,IL-8,GM-CSF and TNF-αinduced by LPS was examined by RT-qPCR.Results Compared to the control,the LPS treatment of human monocytes THP-1 at 1μg/mL for 24 h could significantly increase the expression of IL-1β,IL-6,IL-8,GM-CSF and TNF-α(P<0.05).Treatment with baicalin at 10,30 and 100μmol/L on human monocytes THP-1 for 24 h and 48 h showed no significant cell cytotoxicity.The baicalein at 30μmol/L and 100μmol/L could significantly inhibit the LPS-induced increasing expression of IL-1β,IL-6,IL-8,GM-CSF and TNF-αin human monocytes THP-1.Conclusion The baicalin could significantly inhibit LPS-induced inflammatory response in human monocytes THP-1.

关 键 词：黄芩苷 脂多糖 人单核细胞 炎症反应 细胞因子 

分 类 号：R76[医药卫生—耳鼻咽喉科]