珊瑚菜抗盐相关基因GlERF11克隆与盐胁迫表达分析  

Cloning and Expression Analysis of Salt Resistance-Related Gene Gl ERF11 in Glehnia littoralis

作　　者：徐瑶 任宏伟 孙唯航 张馨方 田雪梅[1] 谭玲玲[1] 袁涛 高婷[1] Xu Yao;Ren Hongwei;Sun Weihang;Zhang Xinfang;Tian Xuemei;Tan Lingling;Yuan Tao;Gao Ting(Shandong Provincial University Key Laboratory of Plant Biotechnology,School of Life Sciences,Qingdao Agricultural University,Qingdao 266109,China;College of Civil Engineering&Architecture,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学生命科学学院/山东省高校植物生物技术重点实验室,青岛266109 [2]青岛农业大学建筑工程学院,青岛266109

出　　处：《世界科学技术-中医药现代化》2022年第5期1875-1884,共10页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会青年基金项目(81903748):黄芩黄酮合成相关R2R3-MYB转录因子可变剪切异构体的功能及机理研究,负责人:高婷;山东省教育厅山东省高校科技计划项目(2019KJE003):山东药用木腐真菌资源多样性研究,负责人:田雪梅。

摘　　要：目的 鉴定珊瑚菜抗盐基因GlERF11并分析其在盐处理下的表达量变化。方法 对GlERF11基因进行cDNA全长克隆以及生物信息学分析,使用荧光定量PCR方法对GlERF11基因进行定量表达分析。结果 GlERF11基因cDNA全长1019 bp,开放阅读框全长537 bp,编码178个氨基酸。生物信息学分析显示,GlERF11蛋白为亲水性蛋白,无跨膜结构域,相对分子质量为19710.95 Da,包含AP2超家族保守结构域。系统进化树分析显示,GlERF11蛋白与伞形科植物胡萝卜ERF11蛋白亲缘关系较近。荧光定量分析显示,珊瑚菜幼苗的GlERF11基因受盐胁迫影响后表达量均有所提高,处理后12 h变化最为显著。结论 本研究针对珊瑚菜抗盐相关基因GlERF11开展生物信息学分析,研究为珊瑚菜品种改良以及育种提供了重要的理论基础与思路。Objective To identity the salt resistance gene GlERF11of Glehnia littoralis, and analyze its expression changes under salt treatment. Methods The full-length cDNA cloning and bioinformatics analysis of GlERF11 gene were carried out, and the quantitative expression of GlERF11 gene was analyzed by fluorescence quantitative PCR.Results The full-length cDNA of GlERF11 gene was 1019 bp, and the full-length open reading frame was 537 bp,encoding 178 amino acids. Bioinformatics analysis showed that GlERF11 protein was a hydrophilic protein without transmembrane domain. Its relative molecular weight was 19710.95 DA and contained the conserved domain of AP2superfamily. Phylogenetic tree analysis showed that GlERF11 protein was closely related to ERF11 protein of Daucus carota in Umbelliferae. Fluorescence quantitative analysis showed that the expression level of this gene increased under NaCl treatment, and the peak was reached after 12 hours of treatment.Conclusion In this study, bioinformatics analysis is carried out for GlERF11, a gene related to salt resistance in Glehnia littoralis. This study provides an important theoretical basis and ideas for variety improvement and breeding of Glehnia littoralis.

关 键 词：珊瑚菜 抗盐基因 生物信息学 表达分析 

分 类 号：R284[医药卫生—中药学]