C57BL/6和ICR品系小鼠在大脑中动脉栓塞(MCAO)模型中差异的研究  

Evaluation of Middle Cerebral Artery Occlusion(MCAO) Model in C57BL/6 and ICR Mice

作　　者：郑晓宇 宋文婷[1] 李磊[1] 曹慧 姚明江[1] 刘建勋[1] Zheng Xiaoyu;Song Wenting;Li Lei;Cao Hui;Yao Mingjiang;Liu Jianxun(Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China;Beijing University of Chinese Medicine,Xiyuan Hospital,Beijing 100029,China)

机构地区：[1]中国中医科学院西苑医院基础医学研究所/北京市中药药理重点实验室,北京100091 [2]北京中医药大学临床医学院西苑医院,北京100029

出　　处：《世界科学技术-中医药现代化》2022年第5期1925-1932,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会重点项目(8203000944):血瘀证发生与演变的血小板系统生物学机制研究,负责人:刘建勋;国家自然科学基金委员会面上项目(81873041):肠道菌群代谢物诱导单核细胞亚群变化在AS中的意义与化痰祛瘀解毒通脉方的干预机制研究,负责人:刘建勋。

摘　　要：目的 探讨C57BL/6与ICR小鼠在大脑中动脉栓塞模型(middle cerebral artery occlusion,MCAO)中的品系间差异。方法 设C57BL/6正常组和ICR正常组为5只,C57BL/6模型组和ICR模型组分别设10只,模型组采用相同的MCAO方法进行造模,观察每组小鼠体重变化率、神经行为学、脑指数、脑含水量、脑梗死面积、生存率。结果 (1)C57BL/6与ICR小鼠分别于术前和脑缺血后24 h进行统计,体重变化率具有显著的统计学差异(P<0.01);(2)C57BL/6与ICR小鼠的生存率分别为90.91%和72.72%;(3)C57BL/6与ICR小鼠的神经行为学评分无显著的统计学差异;(4)C57BL/6与ICR小鼠的脑指数分别为1.91%±1.34%、2.06%±1.76%,两组间的差异具有统计学意义(P<0.05);(5)C57BL/6与ICR小鼠的脑含水量分别为82.32%±1.34%、85.83%±1.76%,两组间具有显著的统计学差异(P<0.01);(6)C57BL/6与ICR小鼠的脑梗死面积分别为13.85%±4.32%、28.18%±8.29%,两组间具有显著的统计学差异(P<0.01)。结论 采用相同的MCAO造模方法均可成功制作大脑中动脉栓塞模型,但在C57BL/6与ICR小鼠间存在较为显著的品系间差异。Objective To explore the differences between C57BL/6 and ICR mice in the middle cerebral artery occlusion(MCAO) model. Methods We set C57BL/6 control group and ICR control group as 5 mice, and C57BL/6model group and ICR model group with 10 mice respectively. The model group was modeled by the same MCAO modeling method. The weight change rate, neurobehavioral, brain index, cerebral water content, cerebral infarction area and survival rate of mice were observed.Results(1)C57BL/6 and ICR mice were counted before operation and 24 h after cerebral ischemia, and the weight change rate was significantly different(P<0.01);(2)The survival rates of C57BL/6and ICR mice were 90.91% and 72.72%, respectively;(3)The neurobehavioral scores of C57BL/6 and ICR mice were not significantly different;(4) The brain index of C57BL/6 and ICR mice were 1.91%±1.34%, 2.06%±1.76%, and the difference between the groups was statistically significant(P<0.05);(5)The brain water content of C57BL/6 and ICR mice was 82.32%±1.34%, 85.83%±1.76%, respectively. There was a significant statistical difference between the groups(P<0.01);(6)The cerebral infarct areas of C57BL/6 and ICR mice were 13.85%±4.32% and 28.18%±8.29%, and there was a significant statistical difference between the groups(P<0.01). Conclusion There are significant differences between C57BL/6 and ICR mice using the same MCAO modeling method.

关 键 词：小鼠 大脑中动脉栓塞模型 C57BL/6 ICR 品系间差异 

分 类 号：R2-03[医药卫生—中医学]