人参皂苷Rg5对膝骨性关节炎模型大鼠软骨细胞和基质的影响  

Efficacy of Ginsenoside-Rg5 on Chondrocytes and Cartilage Matrix in Rats with Knee Osteoarthritis Model

作　　者：樊孝俊[1] 何维佳 叶海程 苏春涛[1] FAN Xiaojun;HE Weijia;YE Haicheng;SU Chuntao(Department of Rehabilitation Medicine,First Affiliated Hospital of Xiamen University,Xiamen 361003,Fujian China)

机构地区：[1]厦门大学附属第一医院康复医学科,福建厦门361003

出　　处：《中国中医骨伤科杂志》2022年第10期13-17,22,共6页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：福建中医药大学校管课题(XB2021088)。

摘　　要：目的:观察人参皂苷Rg5(G-Rg5)对膝骨性关节炎(KOA)大鼠模型的关节软骨细胞、糖胺聚糖(GAG)代谢的影响。方法:采用离断前交叉韧带造模24只膝骨性关节炎模型大鼠,分为空白对照组(NC组)、生理盐水组(NS组)及关节腔注射不同浓度人参皂苷的3μg和10μg G-Rg5组,每周注射2次,重复6周。组织形态学观察采用苏木精-伊红(HE)染色和番茄O-固绿染色,评估改良的Mankin评分(MMS)、软骨细胞计数、软骨厚度及软骨基质糖胺聚糖含量。结果:组织形态学染色可见NS组软骨表面粗糙甚至局部缺损,基质染色不均,软骨细胞簇集,软骨层次不清晰,潮线消失,呈明显骨性关节炎样改变。相比NS组,10μg G-Rg5组软骨表面相对平整,基质染色均匀,潮线存在,软骨细胞数、软骨厚度和糖胺聚糖含量明显升高,MMS明显降低(P<0.05),甚至MMS和糖胺聚糖含量接近NC组(P>0.05)。而3μg G-Rg5组软骨表面不平整,基质染色不均,呈轻度骨性关节炎样特点,全层软骨细胞数和软骨厚度、糖胺聚糖含量无明显升高(P>0.05)。结论:G-Rg5对膝骨性关节炎具有保护作用,可以改善或延缓关节软骨损伤,且呈剂量依赖性抑制膝骨性关节炎模型大鼠的关节软骨细胞凋亡和基质糖胺聚糖含量减少。Objective:To observe the efficacy of ginsenoside-Rg5(G-Rg5) on the metabolism of articular chondrocytes and glycosaminoglycans in rats with knee osteoarthritis(KOA).Methods:24 KOA model rats established by transecting anterior cruciate ligament were divided into the normal control group(NC),the normal saline group(NS),the 3 μg G-Rg5 group and 10 μg G-Rg5 group.All injection was carried out twice a week for 6 weeks.The cartilage morphology was evaluated by hematoxylin-eosin(HE) staining and Safranin O-Fast Green staining.Modified Mankin’s scores(MMS),cartilage glycosaminoglycans(GAG) content, number of chondrocyte and cartilage thickness were evaluated.Results:Histological staining showed that the NS group had rough surface or even local defects, uneven coloration cartilage matrix, chondrocyte clustering, unclear cartilage layers, tidemark disappearance and presenting obvious characteristics of osteoarthritis-like changes.Compared with NS group, 10 μg G-Rg5 group had a relatively smooth cartilage surface, uniform coloration cartilage matrix, present tidemark line and signific improvement of the number of chondrocytes, cartilage thickness and GAG content, and its MMS was significantly decreased(P<0.05).10 μg G-Rg5 group in cartilage MMS and GAG content closed to NC group(P>0.05).However, the 3 μg G-Rg5 group had uneven cartilage surface and uneven coloration cartilage matrix, mild osteoarthritis-like characteristics, and the number of full-layer chondrocytes, cartilage thickness and GAG content in 3 μg G-Rg5 group were not significantly increased(P>0.05).Conclusion:G-Rg5 had a protective efficacy on KOA and can improve or delay articular cartilage injury, and inhibit chondrocyte apoptosis and lose of GAG content of KOA model rats in a dose-dependent manner.

关 键 词：膝骨性关节炎 人参皂苷Rg5 软骨细胞 软骨基质 

分 类 号：R684.3[医药卫生—骨科学]