极地海绵共附生放线菌Streptomyces sp.LHW11-07次生代谢产物研究  

Study on the secondary metabolites from a polar marine sponge associated actinomyces Streptomyces sp.LHW11-07

作　　者：卢静容 刘雅葳 代影 林厚文[1] 杨帆[1] LU Jingrong;LIU Yawei;DAI Ying;LIN Houwen;YANG Fan(Department of Pharmacy,Renji Hospital Affiliated to School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China;School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海交通大学医学院附属仁济医院药学部,上海200127 [2]沈阳药科大学中药学院,辽宁沈阳110016 [3]上海海洋大学食品学院,上海201306

出　　处：《药学实践与服务》2022年第6期499-504,共6页Journal of Pharmaceutical Practice and Service

基　　金：国家自然科学基金项目(82073758)。

摘　　要：目的对极地海绵共附生放线菌Streptomyces sp.LHW11-07进行次生代谢产物的研究,以期得到结构新颖且具有良好生物活性的小分子化合物。方法综合运用凝胶柱色谱、硅胶柱色谱、反相中压柱色谱和高效液相色谱等分离手段对Streptomyces sp.LHW11-07发酵产物进行分离和纯化,采用质谱、一维、二维核磁共振等现代波谱方法并结合相关文献调研鉴定它们的结构。结果从该菌的发酵产物中共分离得到9个单体化合物,分别为cyclo-(L-Tyr-L-Trp)(1)、cyclo-(LTrp-L-Ser)(2)、cyclo-(D-Tyr-D-Pro)(3)、cyclo-(L-Tyr-L-Phe)(4)、cyclo-(L-Tyr-L-Leu)(5)、albaflavenol B(6)、β-adenosine(7)、N-formylantimyic acid methyl ester(8)和conglobatin A(9)。结论化合物1和2是首次分离于Streptomyces属放线菌。Objective In order to obtain small molecule compounds with novel structure and good biological activity,the secondary metabolites of polar sponge-symbiotic Streptomyces sp.LHW11-07 were studied.Methods The fermentation product of Streptomyces sp.LHW11-07 was isolated and purified by gel column chromatography,silica gel column chromatography,reversed-phase medium pressure column chromatography and high performance liquid chromatography.The structures of the monomeric compound were identified by modern spectroscopic methods such as mass spectrometry,nuclear magnetic resonance and related literature reports.Results A total of nine compounds were isolated from the fermentation of this strain,which were cyclo-(L-Tyr-L-Trp)(1),cyclo-(L-Trp-L-Ser)(2),cyclo-(D-Tyr-D-Pro)(3),cyclo-(L-Tyr-L-Phe)(4),cyclo-(L-Tyr-L-Leu)(5),albaflavenol B(6),β-adenosine(7),N-formylantimyic acid methyl ester(8)and conglobatin A(9).Conclusion Compounds 1 and 2 were isolated from Streptomyces sp.for the first time.

关 键 词：极地 海绵 放线菌 次生代谢产物 

分 类 号：R284[医药卫生—中药学]