HPLC法同时测定陈香露白露片中7个成分的含量  

Simultaneous determination of seven constituents in Chenxiang Lubailu tablet by HPLC

作　　者：张璐 戴群芳 陈思思 ZHANG Lu;DAI Qunfang;CHEN Sisi(Nanchang Inspection and Testing Center,Nanchang 330000,China)

机构地区：[1]江西省南昌市检验检测中心,江西南昌330000

出　　处：《药学实践与服务》2022年第6期553-556,570,共5页Journal of Pharmaceutical Practice and Service

摘　　要：目的构建同步测定陈香露白露片中甘草苷、甘草酸铵、橙皮苷、川陈皮素、橘皮素、木香烃内酯和去氢木香内酯含量的HPLC法。方法采用ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸溶液,梯度洗脱,流速1.0 ml/min,柱温35℃,检测波长237 nm(检测甘草苷、甘草酸铵、木香烃内酯、去氢木香内酯)、283 nm(检测橙皮苷)、330 nm(检测川陈皮素、橘皮素),进样量10μl,对采集的16批次样品进行7种成分的含量检测。结果甘草苷、橙皮苷、甘草酸铵、川陈皮素、橘皮素、木香烃内酯、去氢木香内酯的线性范围分别为1.110~55.72(r=0.9992)、22.15~1108(r=0.9995)、6.140~307.2(r=0.9995)、1.130~56.25(r=0.9997)、0.3700~18.75(r=0.9982)、0.5200~26.01(r=0.9991)、1.180~58.95(r=0.9999)μg/ml。回收率(n=9)分别为98.71%、98.12%、98.44%、98.22%、99.17%、99.18%、97.93%,RSD分别为0.16%、0.67%、0.57%、0.62%、0.48%、0.56%、0.58%。16批样品中甘草苷、甘草酸铵、橙皮苷、川陈皮素、橘皮素、木香烃内酯、去氢木香内酯的含量分别为0.1250~1.174、2.354~7.426、1.822~27.21、0.0370~1.399、0.0723~0.4433、0.0140~0.1990、0.2207~1.407 mg/g。结论该方法准确性高、重复性好、耐用性强,可用于陈香露白露片的质量控制和评价。Objective To establish the method for the simultaneous determination of liquiritin,ammonium glycyrrhizinate,hesperidin,nobiletin;tangeretin;costunolide,dehydrocostuslactone in Chenxiang Lubailu tablet by HPLC.Methods ZORBAX Eclipse XDB-C18 chromatographic column(4.6 mm×250 mm,5μm)was used.The mobile phase was methanol-0.1%phosphoric acid solution.Gradient elution with flow rate of 1.0 ml/min was used.Column temperature was 35℃.Detection wavelength for liquiritin,ammonium,tangeretin,and costunolide was at 237 nm.Detection wavelength for glycyrrhizinate was at 283 nm.Detection wavelength for hesperidin and nobiletin was at 330 nm.Injection volume was 10μl.16 batches of samples were tested.Results The linear ranges for the detection of liquiritin,ammonium,glycyrrhizinate,hesperidin,nobiletin,tangeretin,and costunolide were 1.110-55.72(r=0.9992),22.15-1108(r=0.9999),6.140-307.2(r=0.9995),1.130-56.25(r=0.9997),0.3700-18.75(r=0.9982),0.5200-26.01(r=0.9991),and 1.180-58.95(r=0.9999)μg/ml respectively.The average recoveries were 98.71%,98.12%,98.44%,98.22%,99.17%,99.18%,and 97.93%,and the RSDs were 0.16%,0.67%,0.57%,0.62%,0.48%,0.56%,and 0.58%respectively.The contents of the seven components in 16 batches of samples were 0.1250-1.174,2.354-7.426,1.822-27.21,0.0370-1.399,0.0723-0.4433,0.0140-0.1990,and 0.2207-1.407 mg/g respectively.Conclusion The method is accurate,reproducible and durable,which could be used to the quality control and evaluation of Chenxiang Lubailu tablet.

关 键 词：高效液相色谱法 陈香露白露片 甘草苷 甘草酸铵 橙皮苷 川陈皮素 橘皮素 木香烃内酯 去氢木香内酯 

分 类 号：R917[医药卫生—药物分析学]