非洲猪瘟病毒诱导猪红细胞凋亡并促进猪外周血单核细胞的吞噬作用  

African Swine Fever Virus Induces Porcine Red Blood Cells Apoptosis and Promotes Phagocytosis of Porcine Peripheral Blood Mononuclear Cells

作　　者：杨云龙 冯永智 高琦 郑佳琛 王衡 张桂红[1,2,3] 龚浪 YANG Yunlong;FENG Yongzhi;GAO Qi;ZHENG Jiachen;WANG Heng;ZHANG Guihong;GONG Lang(College of Veterinary Medicine,South China Agricultural University/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province,Guangzhou 510642,China;African Swine Fever Regional Laboratory of China(Guangzhou)/Research Center for African Swine Fever Prevention and Control,Guangzhou 510642,China;Maoming Branch of Guangdong Laboratory for Lingnan Modern Agriculture,Maoming 525000,China)

机构地区：[1]华南农业大学兽医学院/广东省动物源性人兽共患病预防与控制重点实验室,广东510642 [2]国家非洲猪瘟区域实验室(广州)/华南农业大学非洲猪瘟防控技术研究中心,广州510642 [3]岭南现代农业科学与技术广东省实验室茂名分中心,茂名525000

出　　处：《畜牧兽医学报》2022年第11期3948-3955,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：广东省重点领域研发计划(2019B020211003);广州市重点领域研发计划(202206010036);国家自然科学基金(31941004);茂名实验室科研启动项目(2021TDQD002);财政部和农业农村部:国家现代农业产业技术体系基金(CARS-35)。

摘　　要：本研究旨在探讨非洲猪瘟病毒(ASFV)对猪红细胞(RBCs)的作用以及对猪外周血单核细胞(PBMs)吞噬能力的影响。试验采用流式细胞术检测ASFV侵染猪原代肺泡巨噬细胞(PAMs)和猪红细胞(RBCs)的能力,并检测ASFV诱导RBCs发生凋亡的百分比;同时采用激光共聚焦试验(Confocal)观察ASFV诱导RBCs发生凋亡是否影响PBMs的吞噬能力。结果显示,ASFV不能入侵RBCs,但以时间和剂量依赖性方式诱导RBCs发生凋亡。0.1 MOI ASFV接种RBCs后1、3、5和7 d可分别诱导1.27%、3.23%、7.39%和8.56%的RBCs发生凋亡;1 MOI ASFV接种RBCs后1、3、5和7 d可分别诱导1.54%、3.73%、8.46%和10.74%的RBCs发生凋亡;3 MOI ASFV接种RBCs后1、3、5和7 d可分别诱导2.65%、5.01%、12.44%和18.61%的RBCs发生凋亡。同时,凋亡的RBCs可以增加PBMs对黄绿色荧光微球的吞噬数量,ASFV诱导RBCs凋亡的百分比越高,PBMs吞噬黄绿色荧光微球的数量越多。综上所述,ASFV不能侵染猪RBCs,但可以以时间和剂量依赖性方式诱导RBCs发生凋亡并增强PBMs的吞噬功能。The purpose of this study was to investigate the effect of African swine fever virus(ASFV) on porcine red blood cells(RBCs) and its effect on the porcine peripheral blood mononuclear cells(PBMs).Flow cytometry was used to detect the ability of ASFV to infect porcine primary alveolar macrophages(PAMs) and RBCs,and the percentage of ASFV-induced apoptosis of RBCs was detected.Whether induction of RBCs apoptosis affects the phagocytic ability of PBMs.The results showed that ASFV could not invade erythrocytes,but induced apoptosis of RBCs in a time-and dose-dependent manner.Apoptosis of RBCs at 1,3,5 and 7 days after inoculation with varying MOI(0.1,1,3) ASFV were as follows:at the MOI of 0.1,the data were 1.27%,3.23%,7.39% and 8.56%,respectively;at the MOI of 1,the data were 1.54%,3.73%,8.46% and 10.74%,respectively;at the MOI of 3,the data were 2.65%,5.01%,12.44% and 18.61%,respectively.At the same time,apoptotic RBCs can increase the number of yellow-green fluorescent microspheres phagocytosed by PBMs.In conclusion,ASFV cannot invade porcine RBCs,but ASFV induces RBCs apoptosis and promotes the phagocytic ability of PBMs in a time-and dose-dependent manner.

关 键 词：非洲猪瘟病毒 猪红细胞 细胞凋亡 吞噬 

分 类 号：S852.659.1[农业科学—基础兽医学]