C型产气荚膜梭菌实验感染仔猪肠道circRNA的表达特征  

Characterization of circRNA Expression Profiles Involved in Intestines of Clostridium Perfringens Type C-infected Diarrheal Piglet

作　　者：黄晓宇[1] 杨巧丽 闫尊强 王鹏飞[1] 石海仁 滚双宝[1,3] HUANG Xiaoyu;YANG Qiaoli;YAN Zunqiang;WANG Pengfei;SHI Hairen;GUN Shuangbao(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Tibet Academy of Agricultural and Animal Husbandry Science,Lhasa 850000,China;Gansu Research Center for Swine Production Engineering and Technology,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]西藏自治区农牧科学院,拉萨850000 [3]甘肃省现代养猪工程技术研究中心,兰州730070

出　　处：《畜牧兽医学报》2022年第11期4058-4070,共13页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：甘肃省青年基金项目(20JR5RA005);国家自然科学基金(31960646);甘肃农业大学人才专项(GAU-KYQD-2018-27)。

摘　　要：circRNAs在病原菌感染宿主的肠道疾病发展中具有重要的调控作用。C型产气荚膜梭菌(C.perfringens type C)是引起仔猪腹泻及相关肠道炎症的主要细菌之一,对产业造成了严重的经济损失。然而,目前有关circRNAs如何调控仔猪C型产气荚膜梭菌性腹泻的全面且系统的研究未见报道。本研究通过RNA高通量测序研究分析了C型产气荚膜梭菌感染的7日龄仔猪回肠组织circRNAs的表达谱,筛选差异表达circRNAs,并进行差异表达基因GO和KEGG功能富集分析,利用miRanda等软件预测circRNA的microRNA靶点,构建circRNA-miRNA-mRNA的互作网络,并进行qPCR验证。结果显示,在C型产气荚膜梭菌感染的处理组(TI组)和对照组(CI组)中共鉴定出3162个circRNAs,其中差异表达circRNAs有694个,上调表达circRNAs有404个,下调表达circRNAs有290个。功能富集分析结果表明,差异表达circRNAs的亲本基因主要富集在细胞周期、TGF-β信号通路、赖氨酸降解、Wnt信号通路、T细胞受体信号通路、MAPK信号通路等,调节仔猪对产气荚膜梭菌感染的抗性反应。此外,本研究构建了与C型产气荚膜梭菌感染致仔猪腹泻相关的circRNA-miRNA-mRNA互作网络,发现8 circRNAs-5 miRNAs-12 mRNAs组成的ceRNA网络与产气荚膜梭菌感染性疾病密切相关。本试验可为深入研究circRNA调控仔猪C型产气荚膜梭菌性腹泻疾病及猪抗腹泻病品系培育提供参考。circRNAs have important regulatory roles in enteric disease development of host infected by pathogenic bacteria.Clostridium perfringens type C(C.perfringens type C) is one of primary bacteria leading to a series of diarrhea and inflammatory diseases in piglets,which caused serious economic losses over the industry.However,the comprehensive and systematic understanding of circRNAs regulating piglet diarrhea caused by C.perfringens type C has not been reported.To illustrate the dynamics of piglet after C.perfringens infection,this study has investigated the expression profiles of ileum circRNAs of 7-day-old piglets challenged by C.perfringens type C through RNA sequencing,screened the differentially expressed circRNAs,and performed the GO and KEGG functional enriched functions.The targets of circRNAs were predicted by miRanda software,then constructing the circRNA/miRNA/mRNA interaction network and finally conducting the qPCR verification.A total of 3 162 circRNAs were identified with quantitative 694 differentially expressed circRNAs in treatment group(TI) and control group(CI) through sequencing,while 404 of which were up-regulated and 290 were down-regulated.Function analysis revealed that these dysregulated circRNAs were mostly enriched in several KEGG pathways including cell cycle,TGF-beta signaling pathway,Lysine degradation,Wnt signaling pathway,T cell receptor signaling pathway,MAPK signaling pathway and so on,regulating piglets resistance response to C.perfringens infection.Furthermore,the interactive network of circRNA-miRNA-mRNA were built in the C.perfringens type C-infected piglet ileum tissues,and the circRNA-associated ceRNA networks composed of 8 circRNAs-5 miRNAs-12 mRNAs was predicted to have important association with CAED.This study may provide valuable information for understanding the regulatory functions of circRNAs of piglet defensed to C.perfringens type C.

关 键 词：仔猪腹泻 C型产气荚膜梭菌 circRNA 炎性反应 抗性 

分 类 号：S852.616.3[农业科学—基础兽医学]