Reverse genetics in virology:A double edged sword  

作　　者：Hongyu Chen Hongqi Liu Xiaozhong Peng 

机构地区：[1]Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical School,Kunming 650118,China [2]Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical School,Beijing 100051,China [3]Institute of Laboratory Animals Science,Chinese Academy of Medical Sciences and Peking Union Medical School,Beijing 100021,China

出　　处：《Biosafety and Health》2022年第5期303-313,共11页生物安全与健康（英文）

基　　金：Yunnan Key R&D project(202103AQ100001).

摘　　要：Reverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application.However,while reverse genetics has contributed significantly to our understanding of molecular biology and the pathogenesis of viruses,its accessibility(operation)and openness(data)have raised many concerns regarding biosafety and biosecurity.In this review,we retrospectively examine the development of reverse genetics and its applications in virology,then emphasize global biosafety and biosecurity concerns regarding reverse genetics,and summarize global regulations,governance,and laws on reverse genetics.This review seeks to enhance our understanding and rational application of reverse genetics technology for the benefit of humankind.

关 键 词：Reverse genetics VIROLOGY Dual use research of concern BIOSAFETY BIOSECURITY REGULATION 

分 类 号：R373[医药卫生—病原生物学]