基于GC-MS的补阳还五汤改善D-gal模型小鼠学习记忆的血浆代谢组学研究  

Plasma metabolomics study of Buyang Huanwu Decoction improving learning andmemory in D-gal model mice based on GC-MS

作　　者：于明慧 薛傲 赵德萍 徐艳明[1,2] 薛慧 姜晶 孙慧峰[1] 张宁[1,2] YU Ming-hui;XUE Ao;ZHAO De-ping;XU Yan-ming;XUE Hui;JIANG Jing;SUN Hui-feng;ZHANG Ning(College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;College of Jiamusi,Heilongjiang University of Chinese Medicine,Jiamusi 154007,China;Heilongjiang Provincial Institute of Drug Inspection,the Research Institute for Biosafety Inspection,Harbin 150088,China)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007 [3]黑龙江省药品检验研究院生物安全检验研究所,黑龙江哈尔滨150088

出　　处：《海南医学院学报》2022年第22期1681-1687,共7页Journal of Hainan Medical University

基　　金：国家自然科学基金面上项目(82174007);中央支持地方高校改革发展资金人才培养项目;黑龙江省卫生健康委科研课题(2020-303);国家自然科学基金青年项目(82003975);黑龙江中医药大学基金项目(2018RCD19,2018pt04)。

摘　　要：目的:通过GC-MS代谢组学方法,研究了补阳还五汤对D-gal致衰老模型小鼠学习记忆能力的保护效果。方法:选择了24只3月龄的昆明动物作为实验样本,按体重大小随机分成空白组、模型组、补阳还五汤组,通过新体识别试验检测实验动物的记忆水平,HE染色检测动物神经元结构等。采用气相色谱-质谱联用仪对各组实验动物血浆进行了全面定量分析,并利用血浆的代谢过程追溯了病变发生进程中的重要代谢物和主要代谢途径。结果:在HE染色中,与空白组比较,模型组神经元排列异常,形态改变;与模型组比较,补阳还五汤组神经元排列规则,形态正常。在新物体识别实验中,模型组小鼠新物体识别指数降低;补阳还五汤组小鼠新物体识别指数升高。通过GC-MS技术,发现了5种AD的血浆差异代谢产物,分别是L-焦谷氨酸、赖氨酸、焦磷酸、肌酸酐、α-乳糖。结论:补阳正五汤对D-gal模型小鼠疗效显著,并能有效提高其学习记忆的水平。其作用机制可能与谷胱甘肽代谢以及氨酰生物合成等相关代谢通路有关。Objective:To study the protective effect of Buyang Huanwu Decoction on the learning and memory ability of D-gal induced aging mice using GC-MS metabolomics method.Methods:Twenty-four three-month-old kunming animals were selected as experimental samples and randomly divided into control group,model group and Buyang Huanwu Tang group according to their body weight.The memory level of experimental animals was detected by novel body recognition test,and the neuron structure of experimental animals was detected by HE staining.The plasma of each group of experimental animals was quantitatively analyzed by gas chromatography-mass spectrometry,and the important metabolites and main metabolic pathways in the process of pathological changes were traced by using plasma metabolism.Results:In HE staining,compared with blank group,hippocampal neurons in model group were disordered and morphologically abnormal.Compared with the model group,the hippocampal neurons in Buyang Huanwu Decoction group arranged regularly and had normal morphology.Compared with blank group,the index of new object recognition in model group was significantly decreased(P<0.05);Compared with model group,the new object recognition index of Buyang Huanwu Decoction group was significantly increased(P<0.05).Five different metabolites of AD were identified by GC-MS,which were L-pyroglutamate,lysine,pyrophosphoric acid,creatinine andα-lactose.Conclusion:Buyang huanwu Decoction has a significant effect on D-gal model mice,and can effectively improve their learning and memory level.The mechanism may be related to glutathione metabolism and aminophyl biosynthesis.

关 键 词：补阳还五汤 阿尔茨海默病 GC-MS 血浆代谢组学 

分 类 号：R285.5[医药卫生—中药学]