印度梨形孢对百香果抗寒性的影响  

Effect of Piriformospora indica on the cold resistance of passion fruit

作　　者：梁党弟 黄旭萍 唐启文 韦晓霞[3] 李斌奇 Ralf Oelmüller 陈发兴[1] LIANG Dangdi;HUANG Xuping;TANG Qiwen;WEI Xiaoxia;LI Binqi;RALF Oelmüller;CHEN Faxing(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Zhangping Xinqiao Town People′s Government,Longyan,Fujian 364403,China;Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;Matthias Schleiden Institute for Plant Physiology,Friedrich Schiller University Jena,Jena,Thuringia 07743,Germany)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]漳平市新桥镇人民政府,福建龙岩364403 [3]福建省农业科学院果树研究所,福建福州350013 [4]耶拿大学普通植物与植物生理研究所,图林根州耶拿07743

出　　处：《福建农林大学学报（自然科学版）》2022年第6期748-753,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省科技厅农业引导性重点项目(2020N0004);福建省科技厅星火计划项目(2020S0056);现代设施农业福建省高等学校工程研究中心开放基金(G2-KF2001)。

摘　　要：为研究内生真菌印度梨形孢对百香果抗寒性的影响,以‘福建百香果3号’为材料,将印度梨形孢菌液浇灌于百香果幼苗根系,待其定殖30 d后对幼苗进行0℃低温处理,分别在处理0、6、12和24 h时测定幼苗的生理生化指标.结果表明,印度梨形孢显著提高了百香果幼苗叶片的抗氧化酶活性和清除活性氧的能力.与对照组相比,低温胁迫12 h后,接种印度梨形孢的百香果幼苗叶片的过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性和还原型谷胱甘肽(GSH)含量分别提高了60.16%、28.27%、21.31%和23.23%,而多酚氧化酶(PPO)活性、过氧化氢(H_(2)O_(2))浓度和超氧阴离子(O_(2)^(-·))浓度分别降低了81.06%、9.96%和21.96%;低温处理24 h后,接种印度梨形孢的百香果幼苗叶片的超氧化物歧化酶(SOD)活性显著升高(18.18%).可见,印度梨形孢共生植株的细胞膜稳定、抗氧化能力强,说明该内生真菌能够提高种苗的抗寒性.To study the effect of endophytic fungus Piriformospora indica on the cold resistance of passion fruit,the roots of passion fruit seedlings(Fujian Passion Fruit No.3)were infected with P.indica for 30 d,then the seedlings were analyzed for physiological and biochemical indexes after cold treatment at 0℃for 0,6,12 and 24 h,respectively.The results showed that P.indica colonization significantly improved the activities of antioxidant enzymes and the ability of scavenging reactive oxygen species(ROS)in passion fruit leaves.In comparison with the control without P.indica,the activities of catalase(CAT),ascorbate peroxidase(APX),glutathione reductase(GR),and reduced glutathione(GSH)level in leaves were increased by 60.16%,28.27%,21.31%and 23.23%,respectively after cold treatment for 12 h,while the activity of polyphenol oxidase(PPO),hydrogen peroxide(H_(2)O_(2))and superoxide anion radical(O_(2)^(-·))contents were decreased by 81.06%,9.96%and 21.96%,respectively.After underwent cold stress for 24 h,the activity of superoxide dismutase(SOD)was significantly improved by 18.18%.Overall,the symbiosis with P.indica significantly improved the cell membrane stability and antioxidant capacity of passion fruit seedlings under cold stress,indicating the contribution of endophytic fungus on boosting the cold resistance of the seedlings.

关 键 词：百香果 印度梨形孢 抗寒性 苗期 

分 类 号：S663.9[农业科学—果树学]