基于内滤效应的铜纳米团簇灵敏检测亮蓝色素的研究  

Sensitive detection of brilliant blue pigment in copper nanoclusters based on inner filter effect

作　　者：辛成洁 刘海舰 李贞相 刘莹 马凝 XIN Cheng-jie;LIU Hai-jian;LI Zhen-xiang;LIU Ying;MA Ning(College of Chemical Engineering and Environment,Weifang University of Science and Technology,Weifang 262700,China;Weifang Key Laboratory of Pollution Control and Resource Utilization of Chemical Wastewater,Weifang University of Science and Technology,Weifang 262700,China)

机构地区：[1]潍坊科技学院化工与环境学院,山东潍坊262700 [2]潍坊市化工废水污染控制与资源化重点实验室,山东潍坊262700

出　　处：《化学研究与应用》2022年第11期2647-2652,共6页Chemical Research and Application

基　　金：山东省自然科学基金项目(ZR2019MEM046)资助;潍坊科技学院高层次人才科研启动项目(2018RC004)资助。

摘　　要：该研究利用亮蓝(BB)与铜纳米团簇(Cu NCs)之间的荧光内滤效应(IFE),建立了一种快速、灵敏检测亮蓝的方法。室温下,在水溶液中,以牛血清白蛋白(BSA)为封端剂,水合肼(N_(2)H_(4)·H_(2)O)为还原剂,将Cu^(2+)还原为牛血清白蛋白-铜纳米团簇(BSA-Cu NCs),把制备的BSA-Cu NCs作为荧光剂用于检测亮蓝。通过研究发现BB的吸收光谱和BSA-Cu NCs的发射光谱有很好的重叠,当BB和BSA-Cu NCs混合时,BSA-Cu NCs的荧光被显著地淬灭,且淬灭的程度与BB的浓度呈现非常好的线性关系。据此开发了一种荧光传感器,其检测的线性范围为0.015~5μM,检测限为0.015μM,并在实际应用中展现出了很好的前景。In this paper,a fast and sensitive new method for detecting brilliant blue was established by using the inner filter effect(IFE)between brilliant blue(BB)and copper nanoclusters(Cu NCs).In aqueous solution,the Cu^(2+) were directly reduced to bovine serum albumin-copper nanoclusters(BSA-Cu NCs)at room temperature using bovine serum albumin as the end-sealant.It was found that the absorption spectrum of BB and the emission spectrum of BSA-Cu NCs overlapped greatly.When BB was mixed with BSA-Cu NCs,we found that the fluorescence of BSA-Cu NCs was significantly quenched,and the quenching degree had a good linear relationship with the concentration of BB.Based on this,a fluorescence sensor has been developed with a linear range of 0.015~5μM and a detection limit of 0.015μM,which shows a good prospect in practical application.

关 键 词：亮蓝 铜纳米团簇 内滤效应 荧光淬灭 

分 类 号：O657.3[理学—分析化学]