柔肝方对肝纤维化小鼠肝脏内IL-22分泌和IL-22R1表达作用的研究  

Study on effects of Rougan Formula on IL-22 secretion and IL-22R1 expression in liver of mice with liver fibrosis

作　　者：厉姣龙 周振华[1] LI Jiaolong;ZHOU Zhenhua(Shanghai University of Traditional Chinese Medicine,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学,上海中医药大学附属曙光医院,上海201203

出　　处：《上海中医药大学学报》2022年第5期69-76,共8页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81774268);上海市科技创新行动计划临床医学领域项目(19401972500);上海市科技创新行动计划生物医药科技支撑专项项目(21S21900400)。

摘　　要：目的:监测柔肝方对刀豆蛋白A(ConA)联合3,5-二乙氧基羰基-1,4-二氢-2,4,6-三甲基吡啶(DDC)诱导的肝纤维化小鼠肝脏内白介素-22(IL-22)分泌及白介素-22受体1(IL-22R1)表达的影响,探讨柔肝方抗肝纤维化的作用机制。方法:60只小鼠随机分为对照组、模型组和柔肝方组,每组20只。模型组和柔肝方组小鼠尾静脉注射ConA,每周1次,并予DDC饮食;对照组小鼠尾静脉注射等体积0.9%NaCl溶液,正常饮食。柔肝方组小鼠按8.5 g/kg灌胃给予柔肝方颗粒,每天1次;对照组和模型组小鼠灌胃给予等体积0.9%NaCl溶液。药物连续干预10周。10周后处死小鼠,监测各组小鼠肝功能、肝脏中IL-22分泌及IL-22R1表达。结果:(1)与对照组比较,模型组和柔肝方组小鼠肝脏中IL-22、IL-22R1、IL-10R2和STAT3 mRNA表达明显升高(P<0.01,P<0.001);与模型组比较,柔肝方组小鼠肝脏中IL-22和IL-10R2 mRNA表达明显升高(P<0.001)。(2)与对照组比较,模型组肝星状细胞(HSCs)表面的IL-22R1 mRNA表达明显升高(P<0.05)、HSCs内的STAT3 mRNA表达明显升高(P<0.05),柔肝方组HSCs表面的IL-22R1和IL-10R2 mRNA表达明显升高(P<0.05,P<0.001),柔肝方组HSCs内的STAT3 mRNA表达升高(P<0.001);与模型组比较,柔肝方组HSCs表面的IL-22R1 mRNA和HSCs内的STAT3 mRNA表达明显升高(P<0.01,P<0.001)。(3)与对照组比较,模型组和柔肝方组Th22细胞数量明显增加(P<0.001);与模型组比较,柔肝方组Th22细胞数量明显增加(P<0.01)。结论:柔肝方可增加肝脏内IL-22分泌及HSCs表面IL-22R1的表达,从而发挥抗肝纤维化的作用。Objective:To study the effects of Rougan Formula on interleukin-22(IL-22)secretion and IL-22 receptor 1(IL-22R1)expression in the liver of concanavalin A(ConA)combined with 3,5-diethoxy carbonyl-1,4-dihydro-2,4,6-trimethylpyridine(DDC)induced liver fibrosis mice,and to explore the mechanism of Rougan Formula against liver fibrosis.Methods:Sixty mice were randomly divided into control group,model group and Rougan Formula group,with 20 mice in each group. The mice in the model group and Rougan Formula group were injected with ConA through the tail vein once a week and fed with DDC diet. The mice in the control group were injected with equal volume of 0.9% NaCl solution through the tail vein and fed with normal diet. The mice in Rougan Formula group were given Rougan Formula granules by gavage(8.5 g/kg)once a day. The mice in control and model group were given equal volume of 0.9% NaCl solution by gavage. The Drug intervention lasted for 10 weeks. The mice were killed after 10 weeks,liver function,secretion of IL-22 and expression of IL-22R1 in liver were observed.Results:(1) Compared with the control group,the expression of IL-22,IL-22R1,IL-10R2 and STAT3mRNA in the liver of mice in the model group and Rougan Formula group significantly increased(P<0.01,P<0.001). Compared with the model group,the expression of IL-22 and IL-10R2 mRNA in the liver of mice in the Rougan Formula group significantly increased(P<0.001).(2)Compared with the control group,the expression of IL-22R1 mRNA on the surface of hepatic stellate cells(HSCs)in the model group significantly increased(P<0.05),the expression of STAT3 mRNA in HSCs significantly increased(P<0.05),the expression of IL-22R1 and IL-10R2 mRNA on the surface of HSCs in the Rougan Formula group significantly increased(P<0.05,P<0.001),and the expression of STAT3 mRNA in HSCs in the Rougan Formula group significantly increased(P<0.001). Compared with the model group,the expression of IL-22R1 mRNA on the surface of HSCs and STAT3 mRNA in HSCs in the Rougan Formula group significa

关 键 词：柔肝方 肝纤维化 IL-22 IL-22R1 

分 类 号：R285.5[医药卫生—中药学]