腹腔注射右美托咪定后小鼠大脑响应区域初探  

Preliminary study on the response area of mouse brain after intraperitoneal injection of dexmedetomidine

作　　者：李昊[1] 许德娇 岑华蕊 程娟[1] 王烈成[1] Li Hao;Xu Dejiao;Cen Huarui;Cheng Juan;Wang Liecheng(Dept of Physiology,School of Basic Medical Sciences,Anhui Medical University,Hefei 230032;Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518000)

机构地区：[1]安徽医科大学生理学教研室,合肥230032 [2]中国科学院深圳先进技术研究院,深圳518000

出　　处：《安徽医科大学学报》2022年第11期1775-1781,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81971236)。

摘　　要：目的探究腹腔注射右美托咪定(Dex)后小鼠大脑c-Fos细胞表达与相关脑区的催产素(OXT)神经元共标记情况。方法将8~10周18只C57小鼠随机分为Saline组和Dex组。Saline组腹腔注射生理盐水,Dex组腹腔注射Dex(100μg/kg)。腹腔注射120 min后分别对两组小鼠进行灌流取脑,冰冻切片和免疫荧光显微成像。结果免疫荧光c-Fos细胞表达组结果显示:与Saline组相比,Dex组在视丘室上核(SON)、腹外侧视前区(VLPO)、穹窿下器(SFO)、孤束核(SOL)和延髓最后区(AP)中的c-Fos细胞表达增强,差异有统计学意义(P<0.05);在蓝斑(LC)、下丘脑外侧区(LH)、背外侧被盖核(LDT)、臂旁核(PB)、腹侧结节乳头核(TMN)和运动皮层M1/M2中c-Fos细胞表达降低,差异有统计学意义(P<0.05);在视丘室旁核(PVN)、视交叉上核(SCN)中c-Fos细胞表达差异无统计学意义。在PVN、SON脑区中OXT神经元和c-Fos细胞共标记组结果显示:与Saline组相比,Dex组PVN、SON脑区OXT神经元和c-Fos细胞共标记,差异无统计学意义。结论Dex可激活促睡眠等相关脑区,而对促觉醒与运动等相关脑区有一定抑制作用,但并未对PVN、SON脑区中OXT神经元产生影响。Objective To investigate the co-labeling of c-Fos protein expression and oxytocin(OXT)neurons in related brain regions in mice injected with dexmedetomidine(Dex)by intraperitoneal injection.Methods 8-10 week old C57 mice were divided into Saline group and Dex group.The Saline group was intraperitoneally injected with normal saline,the Dex group was intraperitoneally injected with Dex(100μg/kg),the control group was given normal saline(Saline group),and the experimental group was given Dex 100μg/kg(Dex group).120 minutes after intraperitoneal injection,the brains of two groups of mice were collected by perfusion,and thenfrozen section and immunofluorescence microscopy imagingwere performed.Results Immunofluorescence c-Fos protein expression in Dex group and Saline group.The results showed that compared with the Saline group,the Dex group had higher thalamotomy in the supraoptic nucleus(SON),ventrolateral preoptic nucleus(VLPO),subfornical organ(SFO),solitary tract(SOL)and area postrema(AP),and the difference was statistically significant.C-Fos in the locus coeruleus(LC),lateral hypothalamic area(LH),laterodorsal tegmental nucleus(LDT),parabigeminal nucleus(PB),ventral tuberomammillary nucleus(TMN)and motor cortex M1/M2 protein expression decreased,and the difference was statistically significant(P<0.05).There was no difference in c-Fos protein expression in parathalamic nucleus(PVN)and suprachiasmatic nucleus(SCN).The results of the co-labeling group of OXT neurons and c-Fos protein in the PVN and SON brain regions showed that:compared with the Saline group,there was no significant difference in the co-labeling of OXT neurons and c-Fos protein in the PVN and SON brain regions of the Dex group.Conclusion The immunofluorescence co-labeling experiment shows that Dex can activate sleep-promoting and other related brain regions,and has a certain inhibitory effect on wake-promoting and exercise-related brain regions,however,it does not affect the co-labeling of c-Fos protein and OXT neurons in PVN and SON brain region

关 键 词：右美托咪定 免疫荧光 催产素能神经元 

分 类 号：R338.2[医药卫生—人体生理学]