模拟高原环境下实验动物富氧系统的构建及应用  

Construction and application of oxygen enrichment system for experimental animal under simulated hypobaric hypoxia environment

作　　者：崔锦秀 马倩倩 翟明明 张晨旭 刘娟 李远辙 罗二平 汤池 CUI Jinxiu;MA Qianqian;ZHAI Mingming;ZHANG Chenxu;LIU Juan;LI Yuanzhe;LUO Erping;TANG Chi(Department of Military Medical Equipment and Metrology,School of Military Biomedical Engineering,Xi'an 710032,China;College of Life Sciences,Northwest University,Xi'an 710069,China;Shaanxi Provincial Key Laboratory of Bioelectromagnetic Detection and Intelligent Perception,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]空军军医大学军事生物医学工程学系军事医学装备与计量学教研室,陕西西安710032 [2]西北大学生命科学学院,陕西西安710069 [3]空军军医大学陕西省生物电磁检测与智能感知重点实验室,陕西西安710032

出　　处：《空军军医大学学报》2022年第7期822-825,共4页Journal of Air Force Medical University

基　　金：空军装备科研项目(KJ20172A05193);空军军医大学“珠峰工程”军事医学人才扶持基金(RCFCTC2019);陕西省重点研发计划一般项目(2021SF-100)。

摘　　要：目的针对高原弥散富氧研究需求,构建一套动物实验弥散富氧系统,为弥散富氧防护机制研究提供硬件支撑。方法通过检测单元膜在不同压力比条件下的富氧浓度及富氧流量的变化来研究富氧膜元特性;基于该特性组装富氧设备,并在低压低氧舱内测试设备性能;将富氧设备与独立通气笼(IVC)组合构建弥散富氧系统。为验证其有效性,将8周龄雄性SD大鼠随机分为正常(NC)组、低压低氧(HH)组和低压富氧(HO)组。采用低压低氧舱构建等效海拔为5000 m的HH模型,低压低氧舱和本弥散富氧系统共同构建HO模型,而NC组大鼠不作任何干预。NC、HH和HO组造模周期均为7 d。造模完成后,通过苏木精伊红(HE)染色观察大鼠海马结构的变化。结果本研究构建的弥散富氧系统可以在海拔5000 m以下构建≥25%富氧浓度的弥散富氧环境。HE染色结果表明,HH组大鼠海马CA1区锥体神经元变性数目相比NC组明显增多,而HO有效减少了HH导致的锥体神经元变性数目。结论本研究基于膜法空分技术,通过调节压力比装配富氧设备,实现IVC内弥散富氧环境构建,并验证了其防护效果,为下一步进行弥散富氧防护机制研究提供了硬件平台支撑。Objective To establish a diffuse oxygen enrichment system for animal experiment and to provide hardware support for exploring the protective mechanism of diffuse oxygen enrichment,according to the research requirements of diffuse oxygen enrichment at high altitude.Methods The characteristics of oxygen-enriched membrane elements were studied by detecting the changes of oxygen-enriched concentration and oxygen-enriched flow rate of membrane elements under different pressure ratios.Based on the results,the oxygen-enriched equipment was assembled and tested in a hypobaric hypoxia chamber.A diffuse oxygen enrichment system was constructed by combining oxygen-enriched equipment and individual ventilated cage(IVC).To verify its effectiveness,eight-week-old male SD rats were randomly divided into normal control(NC)group,hypobaric hypoxia(HH)group,and hypobaric oxygen-enrichment(HO)group.HH model with an equivalent altitude of 5000 m was established by hypobaric hypoxia chamber,and HO model was established by hypobaric hypoxia chamber and the diffuse oxygen enrichment system,while rats in NC group were not given any intervention.The modeling cycle of NC,HH and HO group was 7 d.After modeling,the changes of hippocampal structure were observed by hematoxylin-eosin(HE)staining.Results The diffuse oxygen enrichment system constructed in this study could construct a diffuse oxygen-enriched environment with an oxygen concentration≥25%below an altitude of 5000 m.The results of HE staining showed a significant increase in pyramidal neuron degeneration in hippocampal CA1 region in HH group compared with NC group,which could be reversed by oxygen-enriched intervention in HO group.Conclusion Based on the membrane air separation,this study achieves the construction of diffuse oxygen-enriched environment in IVC by regulating the pressure ratio to assemble the oxygen-enriched equipment,and verifies its effectiveness,which provides a hardware platform support for further research on the protective mechanism of the diffuse oxygen enric

关 键 词：高原环境 富氧 低压低氧 海马 CA1区 

分 类 号：R318[医药卫生—生物医学工程] Q95-337[医药卫生—基础医学]