Micro-CT评价铁超载影响膝骨关节炎模型小鼠软骨类组织的变化  

Effect of iron overload on cartilaginous tissue in a mouse model of knee osteoarthritis evaluated by micro-CT

作　　者：黎少聪 何琪 潘兆丰 杨均政 陈柏豪 王海彬[2] 周驰[2] Li Shaocong;He Qi;Pan Zhaofeng;Yang Junzheng;Chen Bohao;Wang Haibin;Zhou Chi(Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;First Affiliated Hospital,Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China)

机构地区：[1]广州中医药大学,广东省广州市510405 [2]广州中医药大学第一附属医院,广东省广州市510405

出　　处：《中国组织工程研究》2023年第29期4658-4663,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(82074462),项目负责人:王海彬;广州中医药大学“双一流”与高水平大学学科协同创新团队项目(2021xk53),项目负责人:王海彬。

摘　　要：背景:膝骨关节炎可能与铁代谢异常有关,了解铁超载对膝骨关节炎的作用非常有意义。目的:探究铁超载对膝骨关节炎发病的影响。方法:选取30只7周龄C57B/6J野生型小鼠,采用随机数表法分为正常对照组、手术对照组与手术+右旋糖酐铁组,每组10只。8周龄时,手术+右旋糖酐铁组腹腔注射右旋糖酐铁500 mg/kg,每周1次,注射至18周龄时停止;10周龄时,手术对照组与手术+右旋糖酐铁组行左后膝内侧半月板失稳手术。18周龄时处死所有小鼠,通过Micro-CT检测胫骨微结构参数,番红O-固绿染色观察关节软骨组织病理损伤程度,普鲁士蓝染色证实铁在小鼠关节中的沉积。结果与结论:①Micro-CT检测显示,与正常对照组相比,手术对照组、手术+右旋糖酐铁组骨小梁相对体积、骨小梁厚度、骨小梁数量明显减小(P<0.05),骨小梁分离度、结构模型指数增加(P<0.05);与手术+右旋糖酐铁组比较,手术对照组骨小梁相对体积、骨小梁厚度、骨小梁数量增加(P<0.05),骨小梁分离度、结构模型指数相减小(P<0.05);②番红O染色显示,与正常对照组、手术对照组相比,手术+右旋糖酐铁组小鼠软骨表面缺损、破裂严重,表层软骨细胞数量明显减少,国际骨关节炎研究学会半定量评分更高,提示其关节炎严重程度更高;③普鲁士蓝染色显示,手术+右旋糖酐铁组小鼠滑膜中可见大量被染成深蓝色的铁沉积颗粒,正常对照组与手术对照组均无明显铁沉积颗粒;④结果显示,铁超载能够加重膝骨关节炎的严重程度,并且增加软骨蛋白多糖成分的丢失和软骨下骨的骨丢失,明确了铁超载与膝骨关节炎的关系。BACKGROUND:Knee osteoarthritis may be related to abnormal iron metabolism,and it is very meaningful to understand the effect of iron overload on knee osteoarthritis.OBJECTIVE:To explore the effect of iron overload on the incidence of knee osteoarthritis.METHODS:Thirty 7-week-old C57B/6J wild-type mice were selected and randomly divided into a normal control group,an operation control group,and an operation+iron dextran group,with 10 mice in each group.At the age of 8 weeks,the operation+iron dextran group received intraperitoneal injection of iron dextran 500 mg/kg,once a week,and the injection was terminated at the age of 18 weeks.At the age of 10 weeks,the operation control group and the operation+iron dextran group received left posterior knee medial meniscus instability surgery.All mice were sacrificed at 18 weeks of age.Tibial microstructure parameters were detected by micro-CT.Safranin O-fast green staining was used to observe the degree of pathological damage of articular cartilage.Prussian blue staining confirmed the deposition of iron in mouse joints.RESULTS AND CONCLUSION:(1)Micro-CT test showed that compared with the normal control group,the relative trabecular volume,trabecular thickness and trabecular number of subchondral bone were significantly decreased(P<0.05),and the trabecular separation and structural model index were increased(P<0.05)in the operation control group and operation+iron dextran group.Compared with the operation+iron dextran group,relative trabecular volume,trabecular thickness and trabecular number were increased(P<0.05);trabecular separation and structural model index were decreased(P<0.05)in the operation control group.(2)Safranine O staining showed that compared with the normal control group and the operation control group,the subchondral bone of the operation+iron dextran group had serious defects and ruptures on the surface of cartilage,and the number of surface chondrocytes decreased significantly.The higher Osteoarthritis Research Society International score indicated the

关 键 词：膝骨关节炎 铁超载 MICRO-CT 膝关节 番红-O染色 普鲁士蓝染色 软骨下骨 OARSI评分 

分 类 号：R459.9[医药卫生—治疗学] R313[医药卫生—临床医学] R681