长链非编码RNA调控膝骨关节炎中软骨下骨稳态的作用与机制  

Role and mechanism by which long non-coding RNAs regulate subchondral bone homeostasis in knee osteoarthritis

作　　者：韦宗波 苏允裕[2] 章晓云[1] 黄为 许航 刘荣发 Wei Zongbo;Su Yunyu;Zhang Xiaoyun;Huang Wei;Xu Hang;Liu Rongfa(Ruikang Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530000,Guangxi Zhuang Autonomous Region,China;Qinzhou First People’s Hospital,Qinzhou 535000,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西中医药大学瑞康附属医院,广西壮族自治区南宁市530000 [2]钦州市第一人民医院,广西壮族自治区钦州市535000

出　　处：《中国组织工程研究》2023年第29期4736-4744,共9页Chinese Journal of Tissue Engineering Research

基　　金：广西自然科学基金青年基金(2020GXNSFBA159053),项目负责人:章晓云;黄有荣桂派中医大师培养项目(桂中医药科教发[2022]6号);广西临床重点专科(创伤外科)建设项目(桂卫医发{2021}17号)。

摘　　要：背景:软骨下骨邻近软骨层,在膝骨关节炎进展机制中的重要作用已得到广泛认可,近年来干预软骨下骨稳态的因素逐渐成为研究热点。而长链非编码RNA作为目前研究的一大热点,其在调控骨稳态方面已凸显出一定的优势与价值。目的:了解长链非编码RNA在软骨下骨稳态中的作用机制,对探索膝骨关节炎的防治新途径具有重要意义。方法:以“长链非编码RNA、膝骨关节炎、软骨下骨、成骨细胞、破骨细胞、骨髓间充质干细胞、信号通路”为中文检索词检索中国知网、万方、维普数据库;以“Long non-coding RNA、Knee osteoarthritis、Subchondral bone、Osteoblasts、Osteoclasts、Mesenchymal stem cells、Signal pathway”为英文检索词检索PubMed、Medline、Embase及Web of Science数据库,检索从各数据库建库至2022年7月的相关文献,根据纳入及排除标准最终纳入65篇文献。结果与结论:①软骨下骨稳态与骨髓间充质干细胞、成骨细胞和破骨细胞的增殖、分化、凋亡,周围细胞因子以及血管新生等密切相关。②长链非编码RNA对影响软骨下骨稳态各因素具有调控作用,主要通过Wnt/β-catenin、TGF-β/BMPs/Smad、PI3K/AKT和SDF-1/CXCR4信号通路调节骨髓间充质干细胞、成骨细胞和破骨细胞增殖、分化以及凋亡,从而促进或是抑制骨形成,此外还可调节细胞因子、抑制血管新生,为平衡软骨下骨稳态提供了理论依据,今后有可能为防治膝骨关节炎软骨下骨硬化提供了潜在的靶点。③长链非编码RNA对调节骨形成、骨吸收以及血管新生具有重要意义,而如何发挥其在调控膝骨关节炎的异常软骨下骨方面的作用,也是今后需关注的重点。④由于在研究中极难获取到正常人软骨下骨样本,目前尚未见有正常人软骨下骨中长链非编码RNA的相关报道,同时也缺乏长链非编码RNA直接作用于软骨下骨的临床研究,对调控软骨下骨稳态的作�BACKGROUND:The important role of subchondral bone adjacent to the cartilage layer in the knee osteoarthritis progression has been widely recognized and the factors interfering with subchondral bone homeostasis are gradually becoming a research hotspot.Current studies have revealed that long-stranded noncoding RNAs have highlighted certain advantages and values in regulating bone homeostasis.OBJECTIVE:To understand the working mechanism of long non-coding RNAs in the subchondral bone homeostasis,thereby providing evidence for exploring new prevention and treatment methods for knee osteoarthritis.METHODS:CNKI,WanFang,VIP,PubMed,Medline,Embase,and Web of Science databases were searched with the search terms of“Long non-coding RNA,Knee osteoarthritis,Subchondral bone,Osteoblasts,Osteoclasts,Mesenchymal stem cells,Signal pathway”in Chinese and English.Literature retrieval time was from inception to July 2022,and a total of 65 articles were included in accordance with the inclusion and exclusion criteria.RESULTS AND CONCLUSION:(1)Subchondral bone homeostasis is closely related to the proliferation,differentiation,and apoptosis of mesenchymal stem cells,osteoblasts and osteoclasts,and peripheral cytokines,and angiogenesis.(2)Long non-coding RNAs can modulate various factors affecting subchondral bone homeostasis,mainly through the Wnt/β-catenin,TGF-β/BMPs/Smad,PI3K/AKT,and SDF-1/CXCR4 signaling pathways,which thereby promote or inhibit bone formation by modulating the proliferation,differentiation and apoptosis of bone marrow mesenchymal stem cells,osteoblasts and osteoclasts.It can also modulate cytokines and inhibit angiogenesis.These findings provide a theoretical basis for balancing subchondral bone homeostasis and a potential target site for the prevention and treatment of subchondral osteosclerosis in knee osteoarthritis.(3)Long non-coding RNAs are important for modulating bone formation,bone resorption,and angiogenesis.How this type of RNAs can play a role in modulating the abnormal subchondral bone in knee

关 键 词：长链非编码RNA 膝骨关节炎 软骨下骨 成骨细胞 破骨细胞 骨髓间充质干细胞 信号通路 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R684.3