黑曲霉SICU-33的筛选、培养条件优化及初步应用  

Screening and Culture Condition Optimization of Aspergillus niger SICU-33 and Its Preliminary Application

作　　者：尼玛扎西 Nimazhaxi(Institute of Resources and Environment,Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet Lhasa 850000,China;State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement,Tibet Lhasa 850000,China)

机构地区：[1]西藏自治区农牧科学院农业资源与环境研究所,西藏拉萨850000 [2]省部共建青稞和牦牛种质资源与遗传改良国家重点实验室,西藏拉萨850000

出　　处：《西藏农业科技》2022年第3期52-58,共7页Tibet Journal of Agricultural Sciences

基　　金：西藏自治区重点研发项目(XZ201901NB07);西藏财政预算内项目(XZNKYZHS-2021)。

摘　　要：从青稞根际土壤中挖掘具有促生抗病作用的真菌菌株,以PGPR真菌为研究对象,对其进行鉴定、培养条件优化及促生效果盆栽验证。以IAA定量测定、拮抗病原菌和溶解无机磷效果为指标筛选具有PGPR功能的菌株,通过ITS测序进行分子生物学鉴定,在单因素试验基础上,利用响应面分析中的Box-Benhnken设计优化培养条件,再通过盆栽试验探究菌株促生效果。结果看出,从青稞根际土壤中分离到一株IAA产量达到52.13 mg/L、能拮抗4种病原菌生长、溶磷圈为2.47 cm的真菌菌株SICU-33,初步鉴定为黑曲霉(Aspergillus niger);优化后的培养条件为pH值7、葡萄糖浓度20 g/L、盐浓度15 g/L以及培养基量125 mL,最终得到菌株的生物量为2.56 g;通过盆栽试验证明该菌株对玉米幼苗有明显促生作用。菌株SICU-33能有效促进玉米幼苗生长,具有一定的应用潜力。To excavate fungal strains with growth-promoting and disease-resistant effects from the rhizosphere soil of highland barley,PGPR fungi were used as the research object to identify them,optimize their culture conditions,and verify their growth-promoting effects in pots. Screen strains with PGPR function using IAA quantitative determination,antagonizing pathogenic bacteria and dissolving inorganic phosphorus effects as indicators.molecular biological identification through ITS sequencing were carried out. Based on single factor experiments,use response surface analysis BoxBenhnken to design and optimize culture conditions. Explore the growth-promoting effects of strains through pot experiments. The results show that one strain was isolated from the rhizosphere soil of highland barley with an IAA yield of 52.13 mg/L,which can antagonize the growth of 4 pathogens.The fungal strain SICU-33 with a phosphate zone of 2.47 cm was initially identified as black Aspergillus niger. The optimized culture conditions were pH 7,glucose concentration 20 g/L,salt concentration 15 g/L,and medium volume 125 mL. Finally,the biomass of strain is 2.56 g. It is proved by pot experiment that the strain has obvious growth-promoting effects on corn seedlings. Strain SICU-33 can effectively promote the growth of maize seedlings and has a certain application potential.

关 键 词：黑曲霉 PGPR 响应面分析 培养基优化 

分 类 号：Q949.32[生物学—植物学] S512.3[农业科学—作物学]