Amino acid permease 6 regulates grain protein content in maize  

作　　者：Tianyi Wang Yunfei Li Yumin Huang Xiaoming Zhao Zhaobin Dong Weiwei Jin Wei Huang 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry,National Maize Improvement Center of China,Beijing Key Laboratory of Crop Genetic Improvement,Key Laboratory of Crop Heterosis and Utilization,Ministry of Education,China Agricultural University,Beijing 100193,China [2]College of Agronomy&Resources and Environment,Tianjin Agricultural University,Tianjin 300384,China [3]Center for Crop Functional Genomics and Molecular Breeding,China Agricultural University,Beijing 100193,China [4]Sanya Institute of China Agricultural University,Sanya 572025,Hainan,China

出　　处：《The Crop Journal》2022年第6期1536-1544,共9页作物学报（英文版）

基　　金：supported by the 2022 Research Program of Sanya Yazhou Bay Science and Technology City(SYND-2022-10 to Wei Huang and SYND-2022-03 to Weiwei Jin)。

摘　　要：Grain protein content(GPC)is an indicator of cereal nutritional quality.Identification of genes involved in the regulation of GPC provides targets for molecular breeding for crop protein quality.We characterized a maize gene encoding the putative amino acid transporter ZmAAP6,a gene expressed mainly in immature seeds,especially in the basal endosperm transfer layer.Total protein and zein contents were decreased in ZmAAP6 null mutants and increased in ZmAAP6 overexpression(OE)lines,consistent with their changed in the size of protein bodies.Metabolic and transcriptomic analysis supported the regulatory role of ZmAAP6 in amino acid transportation.These results suggest that ZmAAP6 functions as a positive regulator of GPC in maize,shedding new light on the genetic basis of GPC regulation.

关 键 词：Grain protein content Amino acid MAIZE Amino acid permease 

分 类 号：S513[农业科学—作物学]