Identifying candidate genes and patterns of heat-stress response in rice using a genome-wide association study and transcriptome analyses  

作　　者：Yingxue Yang Chao Zhang De Zhu Huiying He Zhaoran Wei Qiaoling Yuan Xiaoxia Li Xu Gao Bin Zhang Hongsheng Gao Bo Wang Shuaimin Cao Tianyi Wang Yuhua Li Xiaoman Yu Longbiao Guo Guanjing Hu Qian Qian Lianguang Shang 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,Guangdong,China [2]College of Agriculture,Ludong University,Yantai 264025,Shandong,China [3]China National Rice Research Institute(CNRRI),Chinese Academy of Agricultural Sciences,Hangzhou 311401,Zhejiang,China

出　　处：《The Crop Journal》2022年第6期1633-1643,共11页作物学报（英文版）

基　　金：supported by the Agricultural Science and Technology Innovation Program,Shenzhen Science and Technology Program(KQTD2016113010482651);Projects Subsidized by Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District(RC201901-05);Guangdong Basic and Applied Basic Research Foundation(2019A1515110557);the National Natural Science Foundation of China(31771887);Genome-wide Association Study of High Nitrogen Use Efficiency Related Traits in Rice and Breeding Application(2020-KYYWF-0237)。

摘　　要：Because high temperatures impair rice production,it is desirable to elucidate the regulatory mechanisms involved in rice response to heat stress.The objectives of this study were to identify candidate genes and characterize their response patterns during rice adaptation to high temperatures at the seedling stage.Ten heat-associated quantitative-trait loci were identified in a genome-wide association study.Comparison of transcript abundances in heat-sensitive and heat-tolerant rice pools under heat stress revealed approximately 400 differentially expressed genes.The expression of genes from heatsensitive accessions changed more than those from heat-tolerant accessions under heat stress.Alternative splicing(AS)events responded to heat stress in rice.The types of AS variants significant different between the heat-sensitive and heat-tolerant accessions.Expression patterns differing between the heat-sensitive and heat-tolerant accessions were identified for genes known to be involved in heat stress.We identified eleven genes associated with rice heat stress response.These genes could be pyramided to breed heat-tolerant rice accessions.

关 键 词：Heat stress RICE GWAS Alternative splicing Gene pyramiding 

分 类 号：S511[农业科学—作物学]