板栗Hsp90基因家族的鉴定及在胚珠发育过程中的表达分析  

Identification and expression analysis during ovule development of Hsp90 gene family in Castanea mollissima

作　　者：崔艳红 李玲 白倩[1] 杨清 苏淑钗[1] Cui Yanhong;Li Ling;Bai Qian;Yang Qing;Su Shuchai(Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学林学院,森林培育与保护教育部重点实验室,北京100083

出　　处：《北京林业大学学报》2022年第11期10-19,共10页Journal of Beijing Forestry University

基　　金：国家重点研发项目(2018YFD1000605)。

摘　　要：【目的】热激蛋白(Hsp90)是一种在细菌、植物、动物中均广泛存在的高度保守的蛋白质分子,在植物应激反应和生长发育等过程中发挥着重要的作用。板栗作为我国重要的坚果树种,雌雄花比例不同、授粉受精困难及胚败育等现象致使板栗单产低,严重制约板栗的产量。近期有研究发现Hsp90基因家族参与植物的胚形成,本研究首次对板栗中Hsp90基因家族进行鉴定及表达分析,旨在揭示Hsp90基因家族的功能及其在授粉受精和胚珠发育过程中的作用,进而为提高板栗产量提供理论依据。【方法】利用生物信息学方法对板栗Hsp90基因家族成员进行了鉴定,分析家族成员的理化性质、基因结构、进化关系、启动子元件。基于转录组数据分析CmHsp90基因在板栗胚珠不同发育时期的表达情况。【结果】本研究在板栗中共鉴定到10个CmHsp90基因,该基因家族成员的编码区(CDS)长度范围是2 112~2 481 bp,含有4~22个外显子,编码703~826个氨基酸,相对分子量范围为80.67~94.21 kDa,等电点pI范围介于4.81~5.26;不稳定系数介于36.15~46.75之间,除CmHsp90-6、CmHsp90-4、CmHsp90-9外,其他基因编码的蛋白质相对比较稳定。对CmHsp90基因家族蛋白进行亚细胞定位预测发现:5个成员(CmHsp90-1、CmHsp90-4、CmHsp90-5、CmHsp90-9、CmHsp90-10)定位到了细胞质上,2个成员(CmHsp90-7、CmHsp90-8)定位到了内质网上,其余3个成员分别定位到细胞核(CmHsp90-3)、线粒体(CmHsp90-2)和叶绿体上(CmHsp90-6)上。对CmHsp90基因家族蛋白信号肽预测发现,仅CmHsp90-7和CmHsp90-8存在潜在的信号肽位点。CmHsp90基因家族蛋白的motif、保守结构域基本一致,均包含Hsp90结构域(PF00183)和HATPase_C结构域(PF02518)。基于系统进化树分析,10个CmHsp90家族蛋白被分为5个组。对CmHsp90基因家族启动子区域进行顺式作用元件预测,发现光响应元件是CmHsp90基因家族启动子上的主要调控元件,其�[Objective] Recent studies have found that Hsp90 gene family is related to embryo development,but the details of Hsp90 gene family in chestnut are not clear. The identification and analysis of Hsp90 gene family in chestnut provide a theoretical basis for studying the function of Hsp90 gene family and its role in pollination, fertilization and ovule development, so as to improve the yield of chestnut. [Method] The members of chestnut Hsp90 gene family were identified by bioinformatics. The physical and chemical properties, gene structure, evolutionary relationship and promoter elements of the family members were analyzed. The expression of CmHsp90 genes at different developmental stages of ovule was analyzed based on transcriptome data. [Result] A total of 10 CmHsp90 genes were identified in Chinese chestnut. The length of CDS of 10 CmHsp90 gene family members ranged from 2 112 to 2 481 bp, the number of amino acids encoded ranged from 703 to 826. The relative molecular weight ranged from 80.67 to 94.21 kDa, and the pI ranged from 4.81 to 5.26 kDa. The coefficient of instability ranged from 36.15 to 46.75. Except CmHsp90-6, CmHsp90-4 and CmHsp90-9, the proteins encoded by other genes were relatively stable.According to the subcellular localization prediction of CmHsp90 family protein members, five members(CmHsp90-1, CmHsp90-4, CmHsp90-5, CmHsp90-9 and CmHsp90-10) were located in the cytoplasm, two members(CmHsp90-7 and CmHsp90-8) were located in the endoplasmic reticulum, and the other three members were located in the nucleus(CmHsp90-3), mitochondria(CmHsp90-2) and chloroplast(CmHsp90-6).By predicting the signal peptide of CmHsp90 gene family proteins, it was found that only CmHsp90-7 and CmHsp90-8 had potential signal peptide sites. The motif and protein domains of CmHsp90 gene family proteins were basically the same, including Hsp90 domain(PF00183) and HATPase_c domain(PF02518).Based on phylogenetic tree analysis, 10 CmHsp90 proteins were divided into 5 groups. The cis acting elements in the promoter region of

关 键 词：板栗 热激蛋白 基因家族 胚发育 表达模式 

分 类 号：S792.17[农业科学—林木遗传育种] S664.2[农业科学—林学]