高寒草甸植物根际溶磷菌的筛选鉴定及其溶磷与促生效果  

Screening and Identification of Phosphate-Solubilizing Bacteria in Plant Rhizosphere of Alpine Meadow and Their Effects on Phosphate-Solubilizing and Plant Growth Promotion

作　　者：蔺宝珺 杨文权[2] 赵帅[1] 柴港宁 鱼杨华 武燕茹 韩显忠 李希来[4] 寇建村[1] LIN Bao-jun;YANG Wen-quan;ZHAO Shuai;Chai Gang-ning;Yu Yang-hua;WU Yan-ru;HAN Xian-zhong;LI Xi-lai;KOU Jian-cun(College of Grassland Agriculture,Northwest A&F University,Yangling,Shaanxi Province 712100,China;College of Life Sciences,Northwest A&F University,Yangling,Shaanxi Province 712100,China;Menyuan County Grassland Station,Menyuan,Qinghai Province 810300,China;College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai Province 810016,China)

机构地区：[1]西北农林科技大学草业与草原学院,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100 [3]门源县草原站,青海门源810300 [4]青海大学农牧学院,青海西宁810016

出　　处：《草地学报》2022年第11期3132-3139,共8页Acta Agrestia Sinica

基　　金：国家自然科学基金项目(U21A20191);2021年第一批林业草原生态保护恢复资金自然资源调查监测项目(QHXH-2021-018);中央财政林草科技推广示范项目(青[2021]TG01号);青海省财政林业改革发展资金技术推广项目([2021]001号)资助。

摘　　要：为获得高寒草甸根际土中优良溶磷菌资源,本研究从青海省高寒草甸根际土中筛选了4株溶磷菌,结合16S rRNA基因分析法确定其分类地位,并通过钼锑抗比色法和盆栽试验进行了溶磷与促生效果的研究。结果显示:4株溶磷菌均为假单胞菌属(Pseudomonas sp.),均可形成明显的溶磷圈。在无机磷液体培养基中培养7 d后,4株菌株的磷增量在156.17~511.33μg·mL^(-1)之间;溶磷过程中4株菌均分泌多种有机酸,总有机酸量在522.36~986.69 mg·L^(-1)之间;盆栽试验表明4株菌均能显著增加披碱草(Elymus dahuricus Turcz.)株高和地上部干重;菌株MXSC5,MXSC6和MQC13可使植株全氮、全磷含量增加,且4株菌株对土壤速效氮、速效磷含量也有正向影响。本研究结果将有助于进一步探究溶磷菌对植物的促生作用及微生物菌肥的开发利用。In order to obtain the excellent phosphate-solubilizing bacteria resources in the rhizosphere soil of the alpine meadow,four phosphate-solubilizing bacteria were screened from the rhizosphere soil of an alpine meadow in Qinghai province and identified by 16S rRNA gene analysis.Through the anti-molybdenum antimony colorimetry and pot experiment the effects of dissolving phosphorus and promoting growth were studied.The results showed that the four strains were Pseudomonas.They all could form prominent phosphate solubilizing circles.When cultured in inorganic phosphate liquid medium for 7 days,the phosphorus the four strains released was 156.17~511.33μg·mL^(-1).Various organic acids were secreted by the four strains during phosphate solubilization,and the total organic acid amount ranged from 522.36 to 986.69 mg·L^(-1).Pot experiments showed that four strains could significantly increase the plant height and aboveground dry weight of Elymus dahuricus,and promote the growth of Elymus dahuricus.Strains MXSC5,MXSC6 and MQC13 could increase the contents of total N and total P in plants,and the four strains also all had a positive effect on available N and available P content of soil.The results help to investigate further the role of phosphorus-solubilizing bacteria in the promotion of plant growth and the development and utilization of microbial fertilizers.

关 键 词：高寒草甸 溶磷菌 鉴定 溶磷能力 促生效果 

分 类 号：S154.39[农业科学—土壤学]