黄芩-连翘配伍对脂多糖诱导小鼠急性肺炎模型的保护作用  

Protective effect of Scutellariae Radix-Forsythiae fructus in an acute pneumonia mouse model induced by lipopolysaccharide

作　　者：武振帅 纪鹏[1] 魏彦明[1] 徐誉彰 张晓松[1] 武凡琳 任建明 李琛琛 华永丽[1] 姚万玲 袁子文[1] WU Zhenshuai;JI Peng;WEI Yanming;XU Yuzhang;ZHANG Xiaosong;WU Fanlin;REN Jianming;LI Chenchen;HUA Yongli;YAO Wanling;YUAN Ziwen(College of Veterinary Medicine of Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070

出　　处：《中国实验动物学报》2022年第6期800-809,共10页Acta Laboratorium Animalis Scientia Sinica

基　　金：甘肃农业大学伏羲英才项目(Gaufx-02Y05);甘肃省高校产业支撑项目(2020C-14-1);甘肃农业大学青年导师基金资助项目(GAU-QDFC-2021-04);财政部和农业农村部-国家现代产业技术体系资助(CARS-37);甘肃农业大学横向项目(GSAU-JSYF2021-021)。

摘　　要：目的研究黄芩-连翘不同比例配伍对脂多糖(lipopolysaccharide,LPS)诱导小鼠急性肺炎模型的保护作用差异。方法首先采用加热回流法制备不同配伍比例黄芩-连翘组合物。选取健康KM小鼠144只,随机分为12组(正常对照组、模型组、地塞米松阳性药物组、9个黄芩-连翘不同比例配伍组),每组12只,连续灌服相应药物7 d,正常对照组和模型组给予等量生理盐水;第8天,除正常对照组外,其余组小鼠均采用LPS(10 mg/mL)滴鼻法建立小鼠急性肺炎模型。造模6 h后迅速采样。通过小鼠临床体征、日均体重增长量、采食量、脏器指数、小鼠血清TNF-α、IL-1β和IL-6含量、血常规、肺组织形态学观察及肺损伤病理组织学评分,评估黄芩-连翘不同比例配伍组对小鼠急性肺炎模型的保护作用差异。结果建模后各组小鼠存活率为100%。正常对照组小鼠临床体征正常,呼吸音正常,反应灵敏;模型组小鼠反应迟钝,蜷缩扎堆,听诊发现肺部有啰音。不同给药组较模型组相比,整体情况较好,尤其黄芩-连翘配伍组(1∶1,2∶3,1∶2)活动较多,反应较灵敏。小鼠日均体重增长量、日均采食量、肾指数、肝指数、脾指数和胸腺指数差异不显著(P>0.05);与正常对照组比,模型组肺指数、血清中TNF-α、IL1β和IL-6含量、血常规指标、肺组织损伤病理组织学评分显著升高(P<0.01或P<0.05),小鼠肺出现大量炎性细胞浸润,肺泡壁明显增厚、SFTPC蛋白在肺组织肺泡与肺泡间隔区域表达量显著增加,大量阳性细胞表达SFTPC蛋白;与模型组相比,黄芩-连翘配伍组(3∶1,3∶2,2∶1,1∶3,3∶0,0∶3)的心指数、黄芩-连翘配伍组(1∶2)的肺指数、黄芩-连翘配伍组(1∶1,2∶3,1∶2)小鼠血清中TNF-α、IL-1β和IL-6含量、黄芩-连翘配伍组(1∶1,2∶3,1∶2)血常规指标含量、黄芩-连翘配伍组(3∶2,1∶1,2∶3,1∶2)肺组织损伤评分显著降低(P<0.01或P<0.05),4个黄芩-连�Objective To study the protective effect of various proportions of Scutellariae Radix-Forsythiae fructus in an acute pneumonia mouse model induced by lipopolysaccharide(LPS).Methods The Scutellariae Radix-Forsythiae fructus composition with various compatibility ratios was prepared by the heating reflux method.In total,144 healthy KM mice were randomly divided into 12 groups(normal control,model,dexamethasone positive drug,and nine groups of Scutellariae Radix-Forsythiae fructus at various proportions)with 12 mice in each group.Normal control and model groups were administered the same amount of normal saline and the other groups were administered corresponding drugs for 7 continuous days.On day 8,except for the normal control group,mice in other groups were treated with LPS(10 mg/mL)by dripping a solution into the nasal cavity to establish the acute pneumonia model.Samples were collected quickly at 6 hours after modeling.The protective effects of various proportions of Scutellariae Radix-Forsythiae fructus in the acute pneumonia mouse model were evaluated by clinical signs,average daily weight gain,feed intake,the viscera index,serum TNF-α,IL-1βand IL-6 contents,blood routine index,lung histopathological observation,and lung injury histopathological score.Results After modeling,the survival rate of mice in each group was 100%.The normal control group had normal clinical signs,breath sounds,and a sensitive reaction.Mice in the model group were unresponsive and huddled together with rales in the lungs detected by auscultation.Compared with the model group,the overall situation of the various administration groups was better.In particular,the Scutellariae Radix-Forsythiae fructus combination groups(1∶1,2∶3,1∶2)showed more activity and sensitive reactions.No significant differences were observed in daily body weight increase,daily feed intake,or kidney,liver,spleen,and thymus indexes(P>0.05).Compared with the normal control group,the lung index,serum contents of TNF-α,IL-1βand IL-6,blood biochemical indexe

关 键 词：急性肺炎 脂多糖 黄芩 连翘 配伍 

分 类 号：Q95-33[生物学—动物学]