雌性大鼠吸烟对仔鼠行为学影响的研究  

Effect of smoking on behavior of female rats

作　　者：韩雨 陈晨 王鑫峰 张海阳 陈俞 许德玮 姚铁翼 邵晓云[1,3] 徐绍业 HAN Yu;CHEN Chen;WANG Xinfeng;ZHANG Haiyang;CHEN Yu;XU Dewei;YAO Tieyi;SHAO Xiaoyun;XU Shaoye(Basic Medicine College,Guilin Medical University,Guilin 541004,China;Science Experiment Center,Guilin Medical College,Guilin 541004;Key Laboratory of Brain and Cognitive Neuroscience,Guilin Medical College,Guilin 541004;Department of Clinical Medicine,Guilin Medical University,Guilin 541004)

机构地区：[1]桂林医学院基础医学院,广西桂林541004 [2]桂林医学院科学实验中心,广西桂林541004 [3]桂林医学院脑与认知神经重点实验室,广西桂林541004 [4]桂林医学院临床医学院,广西桂林541004

出　　处：《中国实验动物学报》2022年第6期810-818,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(31660269);广西自然科学基金(2021GXNSFAA075004,2017GXNSFAA198003,2016GXNSFBA380098);广西硕士研究生创新项目(YCSW2022371);国家级大学生创新创业训练计划项目(201810601011);广西高等学校千名中青年骨干教师培育计划资助项目。

摘　　要：目的探讨亲代成年雌鼠香烟烟雾暴露对子代大鼠行为学及大脑皮层坍塌反应调节蛋白1(CRMP1)、死亡相关蛋白激酶1(DAPK1)蛋白表达的影响。方法将18只8周龄健康SD雌性大鼠随机分为烟熏组(9只)和对照组(9只),烟熏组行香烟烟雾暴露构建烟熏模型,对照组正常饲养,烟熏1个月后将其与正常雄鼠合笼并继续烟熏直至分娩。将烟熏组和对照组分娩后的子代大鼠(F1代)分别进行旷场实验、转棒疲劳实验、Morris水迷宫实验等动物行为学检测,分析亲代雌鼠香烟烟雾暴露后对其子代的焦虑情况、运动耐受能力、以及空间学习记忆能力的影响。Western Blot检测不同发育阶段(0、7、21 d和4周)各组子代大鼠的大脑皮层组织中DAPK1和CRMP1蛋白表达情况。结果在旷场实验结果显示,烟熏组F1代子代大鼠在总区域中的运动总距离和平均运动速度明显增加(P<0.05),其中进入中央区的次数、在中央区的运动距离和停留时间均较对照组F1代明显增加(P<0.05);转棒疲劳实验结果发现,烟熏组F1代持续运动时间以及运动距离较对照组均显著减少(P<0.05);Morris水迷宫结果显示,烟熏组和对照组F1代的逃避潜伏期和到达平台的游泳路程无明显差异,但游泳速度较对照组明显下降(P<0.05);烟熏组F1代在目标象限停留时间占比和进入目标象限次数均较对照组明显减少(P<0.05),且平均游泳速度和总游泳路程也明显下降(P<0.01);此外,Western Blot结果表明,烟熏组F1代大鼠脑组织中DAPK1和CRMP1在发育早期(0、7、21 d)的蛋白表达均较对照组下降。结论亲代雌鼠香烟烟雾暴露可能会增加其子代大鼠的焦虑情绪,降低运动耐受能力,并使得其空间记忆能力减退,这可能与子代大鼠脑组织中CRMP1和DAPK1分子的调控有关。Objective To investigate the effects of passive smoking in adult female rats on behavior and expression of collapsin response regulatory protein 1(CRMP1)and death-related protein kinase 1(DAPK1)in the cerebral cortex of offspring.Methods Eighteen 8-week-old healthy female SD rats were randomly divided into the passive smoking group(n=9)and control group(n=9).The passive smoking group was treated with smoke to establish the passive smoking model,whereas the control group was treated normally.After 1 month of smoking,female rats were mated with males and continued smoking until delivery.Offspring(F1 generation)of passive smoking and control groups were evaluated by an open field test,rotary rod fatigue test,and Morris water maze test.These animal behavior tests were conducted to analyze the effects of passive smoking on anxiety,exercise tolerance,and spatial learning and memory abilities of the offspring.Moreover,CRMP1 and DAPK1 protein expression in cerebral cortex tissues of offspring was detected by Western Blot at various developmental stages(0,7,and 21 days and 4 weeks(adult))after birth.Results The open field test showed that the total movement distance and average movement speed of the F1 generation in the passive smoking group were significantly increased in the total area(P<0.05),and the number of entering the central area,and the movement distance and residence time in the central area were significantly increased compared with those of the control(P<0.05).The rotary rod fatigue test showed that the sustained movement time and movement distance of the F1 generation in the passive smoking group were significantly reduced compared with those in the control group(P<0.001).The Morris water maze showed that escape latency of the F1 generation had no significant difference between passive smoking group and control groups,but swimming speed was significantly decreased in the passive smoking group(P<0.001).Compared with the control group,the proportion of time and time of the F1 generation entering the target qua

关 键 词：香烟烟雾暴露 子代大鼠 神经系统 行为学 CRMP1 DAPK1 

分 类 号：Q95-33[生物学—动物学]