应用微卫星技术对KM小鼠种子群体遗传质量进行比较分析  

Population genetic quality analysis of a closed KM mouse colony by microsatellites

作　　者：左琴[1] 魏杰[1] 付瑞[1] 刘佐民[1] 王洪[1] 岳秉飞[1] ZUO Qin;WEI Jie;FU Rui;LIU Zuomin;WANG Hong;YUE Bingfei(National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院,北京102629

出　　处：《中国实验动物学报》2022年第6期819-823,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家重点研发计划(2021YFF0703200)。

摘　　要：目的应用微卫星技术在2013年和2020年对同一个KM小鼠种子群体进行遗传质量检测和分析。方法2013年和2020年分别提取30只KM小鼠DNA,应用30个微卫星标记进行PCR扩增,基因测序后计算等位基因数、杂合度和多态信息含量等参数。结果2013年该群体有95个等位基因,平均杂合度为0.4864,平均多态信息含量(PIC)为0.4418。2020年该群体有122个等位基因,平均杂合度分别为0.5150,平均多态信息含量(PIC)分别为0.4818。结论KM小鼠种子群体具有良好的遗传稳定性和多样性,符合封闭群动物的群体遗传概貌特征。Objective To analyze the population genetic quality of the same closed KM mouse colony in 2013 and 2020 by microsatellites.Methods DNA was extracted from 30 KM mice in 2013 and 2020.Thirty pairs of microsatellites primers were used for PCR amplification and DNA sequencing to evaluate genetic quality by the number of alleles,heterozygosity and polymorphism content.Results Ninety-five alleles were found in 2013,average heterozygosity was 0.4864 and average polymorphism content was 0.4418.There were 122 alleles,average heterozygosity was 0.5150,and average polymorphism content was 0.4818.Conclusions The closed KM mice colony had genetic stability and genetic diversity,which satisfied the genetic characteristics of a closed colony of laboratory animals.

关 键 词：KM小鼠 微卫星标记 封闭群:遗传多样性 

分 类 号：Q95-33[生物学—动物学]