金复康调控免疫衰老抑制肺癌转移  

Jinfukang Inhibits Lung Cancer Metastasis by Regulating Immune Senescence

作　　者：姚望 阙祖俊 姚嘉良 于盼 罗斌[1] 田建辉[1,2] YAO Wang;QUE Zujun;YAO Jialiang;YU Pan;LUO Bin;TIAN Jianhui(Department of Oncology,Longhua Hospital Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Research Center for Cancer,Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]上海中医药大学附属龙华医院肿瘤科,上海200032 [2]上海中医药大学附属市中医医院肿瘤临床医学中心,上海200071

出　　处：《肿瘤防治研究》2022年第11期1134-1138,共5页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(81774166);上海市青年科技英才扬帆计划(20YF1449900)。

摘　　要：目的探讨免疫衰老对肺癌转移的影响,并揭示扶正中药金复康防治肺癌转移的作用机制。方法不同月龄C57BL/6小鼠尾静脉注射Lewis肺癌细胞建立肺移植瘤模型;其中6月龄组小鼠采用金复康连续灌胃42天;观察小鼠肺部肿瘤转移情况;流式细胞术检测小鼠外周血中记忆T细胞、NK细胞比例的变化。结果15月龄和6月龄小鼠较2月龄小鼠肺转移成瘤率显著上升(均P<0.05)。外周血记忆CD4+T细胞丰度与小鼠年龄呈正相关(2月龄vs.6月龄,P=0.041;2月龄vs.15月龄,P=0.041)。NK细胞丰度与年龄呈负相关(2月vs.6月,P=0.009;2月vs.15月,P=0.009)。金复康给药组小鼠的生存期较0.9%NaCl组有延长趋势,但差异无统计学意义,而给药组外周血NK细胞水平较0.9%NaCl组显著升高(P=0.029)。结论免疫衰老可促进肺癌转移发生,金复康延长小鼠生存期可能与延缓免疫衰老和改善NK细胞水平有关。Objective To explore the effect of immune senescence on lung cancer metastasis and reveal the mechanism of Fuzheng traditional Chinese medicine Jinfukang in the prevention and treatment of the metastasis.Methods A lung metastasis model of Lewis lung cancer cells was established in C57BL/6 mice with different ages(15 months,6 months,and 2 months).Mice in the 6-month-old group were given Jinfukang intragastrically for 42 days.Pulmonary metastasis was analyzed by in vivo imaging,anatomical microscopic observation,and HE staining.The proportion of memory T cells and NK cells in peripheral blood was detected by flow cytometry.Results The lung metastatic tumor formation rate of 15-month-old and 6-month-old mice was significantly higher than that of 2-month-old mice(all P<0.05).Abundance of CD4+T cells in peripheral blood was positively correlated with age(2-month-old vs.6-month-old,P=0.041;2-month-old vs.15-month-old,P=0.041;6-month-old vs.15-month-old,P=0.953).The abundance of NK cells was negatively correlated with age(2-month-old vs.6-month-old,P=0.009;2-month-old vs.15-month-old,P=0.009;6-month-old vs.15-month-old,P=0.574).However,the survival time of mice in the Jinfukang group was longer(P>0.05)and the level of NK cells in peripheral blood was significantly higher(P=0.029)than those in the normal saline group.Conclusion Immune senescence can promote the metastasis of lung cancer.The prolongation of the survival time of mice administered with Jinfukang may be related to delaying immune senescence and increasing the number of NK cells.

关 键 词：免疫衰老 肺癌 转移 NK细胞 

分 类 号：R273[医药卫生—中西医结合] R734.2[医药卫生—中医肿瘤科]