以番茄为寄主的香蕉穿孔线虫不同群体的致病性差异  

Pathogenetic divergence of different populations in Radopholus similis on tomato

作　　者：杨思华 赵立荣 唐世侨 杨帅 谢辉[1] 徐春玲[1] YANG Sihua;ZHAO Lirong;TANG Shiqiao;YANG Shuai;XIE Hui;XU Chunling(Research Center of Nematodes of Plant Quarantine/College of Plant Protection,South China Agricultural University,Guangzhou 510642,China;Guangzhou Customs District Technology Center,Guangzhou 510623,China)

机构地区：[1]植物检疫线虫检测与防疫研究中心/华南农业大学植物保护学院,广州510642 [2]广州海关技术中心,广州510623

出　　处：《华中农业大学学报》2022年第6期118-127,共10页Journal of Huazhong Agricultural University

基　　金：广东省自然科学基金面上项目(2021A1515011273);国家自然科学基金面上项目(31071665)。

摘　　要：为了建立一种简单易操作的室内香蕉穿孔线虫侵染寄主的致病性测定体系,本研究在(25±1)℃下,通过室内试管石英砂培养的方法,以金丰1号番茄为寄主,以不同接种量和接种处理时间接种不同种群,检测线虫繁殖率、根系病害严重度和植株生长量,以分析不同种群的致病力差异。结果显示:以50、100、150和200条/株分别接种4个不同种群雌虫(HN6、SZ-FZ、GJ-LY323和DBSR)至30 d苗龄植株上,在接种9、16、23和30 d后进行观察分析,发现香蕉穿孔线虫主要侵染番茄根系的皮层细胞并在根系内发育繁殖;不同接种量和接种时间对番茄的致病存在差异,线虫繁殖率、根系病害严重度随接种量和时间的增加而增加,而植株生长量则减少;在以150条/株接种23 d后,各项指标差异最显著,能区分不同种群间的致病性;对香蕉穿孔线虫8个种群以150条/株接种30 d苗龄植株23 d后,发现其致病力从大到小依次为SZ-SWK>HaiN-YJ>HL-XY>SZ-FZ≥GJ-LY323>DBSR>HN6>ML-HG。以上结果表明番茄是香蕉穿孔线虫的良好寄主,在室内以150条/株接种量接种30 d苗龄的番茄,在接种23 d后分析线虫繁殖率、根系病害严重度和植株生长量3项指标可以评估不同种群的致病力差异。In order to establish a simple and easy method to evaluate pathogenicity of Radopholus similis in laboratory,and to determine the pathogenetic difference of different populations,in this study,different populations of Jinfeng No.1 tomato were inoculated with different inoculation concentration and treatment time at(25±1)℃by using quartz sand culture.The nematode reproduction rate,root disease severity and plant growth were detected.Four different populations of female worms(HN6,SZ-FZ,GJ-LY323 and DBSR)were inoculated with 50,100,150 and 200 worms per plant to 30-day-old plants.After 9,16,23 and 30 days of inoculation,it was found that R.similis developed,propagated,and mainly infected the cortical cells of tomato root system.The pathogenicity on tomato was different with different inoculation dose and inoculation time.The reproduction rate of nematodes and the root disease severity increased with the increase of inoculation amount and time,while plant growth decreased.After inoculation with 150 nematodes per plant for 23 days,the most significant differences were observed in each index,which could distinguish the pathogenicity difference among different populations.Eight populations of R.similis were inoculated to 30-day tomato with 150 nematodes per plant for 23 days,and it was found that the pathogenicity of eight populations was in order of SZ-SWK>HaiN-YJ>HL-XY>SZ-FZ≥GJ-LY323>DBSR>HN6>ML-HG.The results showed that tomato was a good host for R.similis,and pathogenicity of different populations can be evaluated by inoculating 30-day-old tomato with 150 nematode per plant indoors for 23 days and analyzing three indexes of nematode reproduction rate,root disease severity and plant growth.

关 键 词：香蕉穿孔线虫 番茄 寄生性 致病性 病害防控 

分 类 号：S436.412.2[农业科学—农业昆虫与害虫防治]