miR-762与sTREM-1 sFLT-1在急性肺损伤中的相关性研究  

Correlation between miR-762 and sTREM-1 sFLT-1 in Acute Lung Injury

作　　者：宋洋[1] 贺晓楠[2] SONG Yang(Beijing Geriatrics Hospital,Beijing 100095,China)

机构地区：[1]北京老年医院,北京100095 [2]首都医科大学,北京100095

出　　处：《河北医学》2022年第11期1822-1826,共5页Hebei Medicine

基　　金：国家自然科学基金委员会资助项目,(编号:81700383)。

摘　　要：目的:观察miR-762在急性肺损伤患者血浆中的表达并探究其与潜在的价值。方法:收集本医院2018年9月至2021年9月接诊的60例肺损伤患者,并依据临床症状和mMRC评分分为中低危组和高危组。急性生理学及慢性健康状况(Acute physiology and chronic health evaluation,APACHEⅡ)评分分析各组患者的得分;酶联免疫吸附实验(ELISA)检测血浆中人可溶性髓系细胞触发受体-1(Soluble myeloid cell trigger receptor-1,sTREM-1)检测试剂盒、人可溶性FMS样酪氨酸激酶1(Soluble FMS like tyrosine kinase 1,sFlt-1)的含量;实时荧光定量聚合酶链式反应(Real time fluorescence quantitative polymerase chain reaction,RT-qPCR)实验检测血浆中miR-762的表达量。绘制受试者工作特征曲线(Receiver operating characteristic curve,ROC)判断miR-762对肺损伤患者的诊断价值及其截断值。结果:高危组、中低危组患者血浆sTREM-1、sFLT-1的含量也明显高于正常组,miR-762的相对表达量显著高于正常组。与中低危组相比,高危组血浆sTREM-1、sFLT-1的含量及miR-762相对表达量均显著升高。ROC曲线显示,miR-762相对表达量作为预测中低危组患者的曲线下面积(Area under curve,AUC)=0.987,95%置信区间(Confidence interval,CI):(0.9688~1.000),截断值为1.210;预测高危组患者的AUC=1.00,95%CI:(1.000~1.000),截断值为2.396。miR-762与APACHEⅡ得分显著升高,sTREM-1、sFLT-1的含量均呈正相关,相关系数(r)分别为0.386、0.321、0.432。结论:急性肺损伤患者血浆miR-762的表达水平升高与肺损伤病情加重相关,是急性肺损伤的预测标记物。Objective:To observe the expression of miR-762 in plasma of patients with acute lung injury and explore its relationship with potential value.Methods:A total of 60 patients with lung injury admitted to our hospital from September 2018 to September 2021 were collected and divided into medium low risk group and high risk group according to clinical symptoms and mMRC scores.Scores were analyzed with acute physiology and chronic health evaluation(APACHEⅡ).Human soluble myeloid cell trigger receptor-1(sTREM-1)kit and human soluble FMS like tyrosine kinase 1(sFlt-1)enzyme linked immunosorbent assay(ELISA)kit were used to detect the contents of sTREM-1,sFlt-1 in plasma;real time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of miR-762 in plasma.Receiver operating characteristic curve(ROC)was plotted to judge the diagnostic value and cut-off point of miR-762 in patients with lung injury.Results:The contents of sTREM-1 and sFlt-1,the relative expression of miR-762 of high-risk group and middle-low-risk group were all significantly higher than those of normal group.Compared with the middle-low-risk group,the contents of sTREM-1,sFlt-1 and the relative expression of miR-762 were substantially increased in the high-risk group in plasma.ROC curve showed that the relative expression of miR-762 was used to predict the area under curve(AUC)=0.987,95%confidence interval(CI):(0.9688-1.000),and the cut-off point was 1.210;AUC=1.00,95%CI:(1.000-1.000),with a cut-off point of 2.396.The scores of miR-762 and APACHEⅡmarkedly increased,and the contents of sTREM-1 and sFlt-1 were positively correlated.The correlation coefficients(r)were 0.386,0.321,0.432.Conclusion:The high expression of miR-762 in plasma from patients with acute lung injury was related to the aggravation of lung injury,and it was a predictive marker of acute lung injury.

关 键 词：急性肺损伤 miR-762 STREM-1 SFLT-1 

分 类 号：R563[医药卫生—呼吸系统]