基于网络药理学的山豆根抗炎活性成分辨析与靶点预测  

Identification of Anti-Inflammatory Active Components and Target Prediction of Subprostrate Sophora Based on Network Pharmacology

作　　者：周沁阳 赵景云[2] ZHOU Qin-yang;ZHAO Jing-yun(School of Chinese Materia Medical,China Pharmaceutical University,Nanjing 211198,China;Yunnan Institute of Chinese Materia Medical,Kunming 650223,China)

机构地区：[1]中国药科大学中药学院,江苏南京211198 [2]云南省中医中药研究院,云南昆明650223

出　　处：《云南中医中药杂志》2022年第11期37-44,共8页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：云南省科技厅重大科技专项(生物医药2018110509);云南省中医中药研究院院级项目(ZYY2018018)。

摘　　要：目的运用网络药理学的方法探究山豆根抗炎作用机制。方法运用TCMSP、GeneCards、String、DAVID 6.8等平台预测山豆根的抗炎成分,并进行基因功能GO富集分析和KEGG通路富集分析,构建“成分-靶点-通路”网络,并通过PPI分析和拓扑分析筛选抗炎成分的核心靶点,使用Cytoscape软件和R语言进行数据可视化。结果筛选得到15个有类药性且口服吸收良好的山豆根抗炎活性成分,包括异鼠李素、槐果碱和苦参碱等成分。功能富集显示,山豆根抗炎成分主要参与到对凋亡的负调控,对缺氧的反应和对脂多糖的反应等生物学过程中。通路富集分析显示,包括HIF-1信号通路、细胞凋亡通路、Ras信号通路和Toll样受体信号通路等被显著富集。拓扑学分析筛选出5个核心靶点基因作为山豆根抗炎的关键靶点,包括SRC、AKT1和MAPK1等。结论山豆根中抗炎活性成分可能主要通过作用于MAPK1和SRC等蛋白核心靶点,并参与到多个炎症相关生物学过程和通路的调控中,最终发挥抗炎效应。Objective:To study the anti-inflammatory mechanism of Subprostrate Sophora by network pharmacology method.Methods:TCMSP,GeneCards,String,DAVID 6.8 and other platforms were used to predict the anti-inflammatory components of Subprostrate Sophora,and gene function GO enrichment analysis and KEGG pathway enrichment analysis were performed to construct the“component-target-pathway”network.And the core targets of anti-inflammatory components were screened by PPI analysis and topological analysis and the data visualization was made by Cytoscape software and R language.Results:A total of 15 anti-inflammatory components of good oral absorption in Subprostrate Sophora were screened,including isorhamnetin,sophorine and matrine.The functional enrichment showed that the anti-inflammatory components were mainly involved in the negative regulation of apoptosis,response to hypoxia and response to lipopolysaccharide.The pathway enrichment analysis showed that HIF-1 signaling pathway,apoptosis pathway,Ras signaling pathway and Toll-like receptor signaling pathway were significantly enriched.The topological analysis screened five core target genes as the key targets of the anti-inflammatory of Subprostrate Sophora,including SRC,AKT1 and MAPK1.Conclusion:The anti-inflammatory active components in Subprostrate Sophora may play an anti-inflammatory effect by acting on core protein targets such as MAPK1 and SRC,and participating in the regulation of multiple inflammatory related biological processes and pathways.

关 键 词：山豆根 网络药理学 抗炎 活性成分 靶点 

分 类 号：R285.5[医药卫生—中药学]