金刚丸对绝经后骨质疏松模型大鼠NF-κB p65表达及IL-1、IL-6、TNF-α的影响  

Effects of Jingang Pill on Expression of NF-κB p65,IL-1,IL-6 and TNF-α in Postmenopausal Osteoporosis Rats

作　　者：陈杰[1,2] 沈琳玲 戎宽 朱亮亮[1] 叶子丰 匡浩铭 匡建军 CHEN Jie;SHEN Lin-ling;RONG Guang;ZHU Liang-liang;YE Zi-feng;KUANG Hao-ming;KUANG Jian-jun(Hunan University of Traditional Chinese Medicine,Changsha 410208,China;The Affiliated Hospital of Hunan Institute of Traditional Chinese Medicine,Changsha 410006,China;Hunan Institute of Traditional Chinese Medicine and Materia Medica,Changsha 410006,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省中医药研究院附属医院,湖南长沙410006 [3]湖南省中医药研究院,湖南长沙410006

出　　处：《云南中医中药杂志》2022年第11期61-66,共6页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：湖南省省自然科学基金项目(S2019JJMSXM2545)。

摘　　要：目的观察金刚丸对去势骨质疏松大鼠核因子-κB(nuclear factor kappa-B,NF-κB)亚基(p65)蛋白表达及血清中白介素-1(Interleukin-1,IL-1)、白介素-6(Interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)含量的影响。方法选择了56只SD大鼠作为本次实验研究的对象,将其划分为空白组(NtC n=8)、假手术组(SHAM n=8)、去势大鼠组(OVX n=40)。对去势大鼠组使用摘除雌性大鼠两侧卵巢法建造绝经后骨质疏松大鼠模型,通过随机数字表法将该组45只大鼠分为模型组、戊酸雌二醇组、金刚丸低剂量组、金刚丸中剂量组、金刚丸高剂量组,其中每组大鼠数量为8只。连续针对金刚丸高中低剂量组及戊酸雌二醇组给予12周药物治疗,每日给药1次,针对假手术组、空白组、模型组则给予相同体积的蒸馏水。12周给药后分别采用HE染色法观察骨组织形态,ELISA法检测血清IL-1、IL-6、TNF-α含量,Western Blot法针对大鼠股骨NF-κB p65表达结果进行对比分析并得到实验结论。结果HE染色结果显示:模型组骨小梁形态模糊、广泛断裂,骨髓腔增宽,骨细胞排列紊乱,戊酸雌二醇组及金刚丸高中低剂量组骨组织形态明显改善,骨小梁结构紧密,骨外膜排列齐整。相较于空白组和假手术组,模型组血清中IL-1、IL-6、TNF-α含量明显升高(P<0.05),股骨中NF-κB p65水平明显升高(P<0.05)。相较于模型组,金刚丸高中低剂量组血清中IL-1、IL-6、TNF-α含量有较显著减低(P<0.05),股骨中NF-κB p65水平有较显著减低(P<0.05)。结论金刚丸能够有效改善骨组织形态,抑制IL-1、IL-6、TNF-α的产生,从而影响NF-κB信号通路,制约NF-κB p65表达活性,起到抑制破骨细胞活化、防治绝经后骨质疏松症的作用。Objective: To observe the effect of Jingang Pill on the expression of nuclear factor kappa-B(NF-κB) subunit(p65) protein and the content of serum Interleukin-1(IL-1) and Interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in castrated osteoporosis rats. Methods: A total of 56 SD rats were selected and divided into normal control group(NtC n=8), sham operation group(SHAM n=8) and castration group(OVX n=40). A rat model of postmenopausal osteoporosis was established by removing both ovaries of female rats in the castrated rats group. 45 rats in this group were randomly divided into model group, estradiol valerate group, Jingang Pill groups of low, medium and high doses, 8 rats each group. The high, medium and low dose groups of Jingang Pill and estradiol valerate group were given drug treatment for 12 weeks, once a day, and the sham operation group, normal control group and model group were given the same volume of distilled water. After 12 weeks of administration, HE staining was used to observe the morphology of bone tissue, ELISA was used to detect the contents of serum IL-1, IL-6 and TNF-α, and Western Blot was used to compare and analyze the expression of NF-κB p65 in rat femur. Results: The HE staining showed that the morphology of trabecular bone in the model group was blurred and extensively fractured, the bone marrow cavity was widened, and the arrangement of osteocytes was disordered. The morphology of bone tissue in the estradiol valate group and the high, medium and low dose Jingang Pill groups was significantly improved, the trabecular bone structure was tight, and the outer bone membrane was arranged neatly. Compared with that of the normal control group and sham operation group, the serum content of IL-1, IL-6 and TNF-α in model group were significantly increased(P<0.05), and the level of NF-κB p65 in femur was significantly increased(P<0.05). Compared with that of the model group, the content of IL-1, IL-6 and TNF-α in serum of the low, medium and high dose groups were significantly decre

关 键 词：金刚丸 绝经后骨质疏松 核因子ΚBP65 白介素-1 白介素-6 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]