退变腰椎间盘髓核组织HMGB2、LEF-1、Col Ⅱ的表达及相关性  

Expression and clinical significance of HMGB2 and LEF-1 in nucleus pulposus of degenerative lumbar intervertebral disc

作　　者：卢飞 吴宇鹏 姚女兆 LU Fei;WU Yupeng;YAO Nyuzhao(Spine Surgery,the First Affiliated Hospital of Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China)

机构地区：[1]南华大学衡阳医学院附属第一医院脊柱外科,湖南省衡阳市421001

出　　处：《中南医学科学杂志》2022年第6期873-875,共3页Medical Science Journal of Central South China

基　　金：湖南省卫生健康委基金项目(20201957);湖南省临床医疗技术创新引导计划项目(2018SK51611)。

摘　　要：目的探讨腰椎间盘髓核组织高迁移率族蛋白B2(HMGB2)、淋巴样增强因子-1(LEF-1)、Ⅱ型胶原(ColⅡ)mRNA及蛋白表达水平及其临床意义。方法收集腰椎间盘突出症患者手术摘除的髓核组织标本20例,根据术前腰椎MR片Pfirrmann分级标准均分为轻度退变组和重度退变组。qRT-PCR和Western boltting检测两组髓核组织HMGB2、LEF-1、ColⅡ的mRNA及蛋白表达水平,并分析其相关性。结果与轻度退变组比较,重度退变组HMGB2 mRNA及蛋白、COLⅡmRNA降低(P<0.05),LEF-1 mRNA升高(P<0.05)。HMGB2水平与ColⅡ水平呈正相关,与LEF-1水平呈负相关(P<0.05);LEF-1水平与ColⅡ水平呈负相关(P<0.05)。结论HMGB2、LEF-1、ColⅡ在不同退变程度腰椎间盘髓核组织中表达水平不同,提示其可能与椎间盘退变的发生相关。Aim To investigate the expression and clinical significance of high mobility group protein B2(HMGB2),lymphoid enhancer factor-1(LEF-1)and typeⅡcollagen(ColⅡ)mRAN and protein in nucleus pulposus of degenerative lumbar intervertebral disc.Methods 20 specimens of nucleus pulposus from patients with lumbar disc herniation were collected,and divided into mild degeneration group(10 cases)and severe degeneration group(10 cases)according to Pfirmann grading standard of preoperative lumbar MR films.The mRNA and protein expression levels of HMGB2,LEF-1,ColⅡin the lumbar disc nucleus pulposus of the two groups were detected by qRT-PCR and Western blotting,and the correlation between the expression levels was analyzed.Results Compared with mild degeneration group,the expression of HMGB2 mRNA and protein,ColⅡmRNA in severe degenerative group decreased(P<0.05),while the relative expression of LEF-1 mRNA increased(P<0.05).HMGB2 was positively correlated with ColⅡand negatively correlated with LEF-1(P<0.05),and LEF-1 was negatively correlated with ColⅡ(P<0.05).Conclusion The expression levels of HMGB2,LEF-1 and ColⅡin the nucleus pulposus of lumbar intervertebral disc at different degrees of degeneration are different,suggesting that they might be related to the occurrence of intervertebral disc degeneration and predictive indicators of disease progression.

关 键 词：高迁移率族蛋白B2 淋巴样增强因子-1 椎间盘退变 

分 类 号：R681.53[医药卫生—骨科学]