HPLC法测定法匹拉韦的有关物质  

Determination of Related Substances of Favipiravir by HPLC

作　　者：张东娜 宋亚彬 王保刚 窦媛媛 徐力昆 王洪权 ZHANG Dong-na;SONG Ya-bin;WANG Bao-gang;DOU Yuan-yuan;XU Li-kun;WANG Hong-quan(Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China)

机构地区：[1]军事科学院军事医学研究院微生物流行病研究所,北京100071

出　　处：《解放军药学学报》2022年第3期189-192,208,共5页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家科技重大专项资助项目,2015ZX09102022。

摘　　要：目的建立HPLC法测定法匹拉韦有关物质的方法。方法采用Venusil XBP C18(250 mm×4.6 mm,5μm)色谱柱;流动相A为0.15%磷酸二氢铵溶液,流动相B为甲醇,梯度洗脱;流速:1.0 ml·min^(-1);检测波长:230和290 nm;柱温:30℃;进样量:10μl。结果法匹拉韦与有关物质能很好地分离,所建立的有关物质检查方法经验证,专属性、重复性及稳定性均良好。结论本研究建立的方法快速准确、重现性好,可用于法匹拉韦有关物质的测定。Objective To establish an HPLC method for determination of related substances in favipiravir.Methods Venusil XBP C18(250 mm×4.6 mm,5μm)was adopted.Mobile phase A consisted of 0.15%ammonium dihydrogen phosphate buffer solution,while mobile phase B was composed of methanol at a flow rate of 1.0 ml·min^(-1).Results The column temperature was 30℃,and the detection wavelength was 230 and 290 nm respectively.Conclusion This method is highly sensitive,specific,accurate and repeatable,so it is a proper method for quality control of favipiravir.

关 键 词：法匹拉韦 广谱抗病毒药 高效液相色谱法 

分 类 号：R943[医药卫生—药剂学]