实验性脂肪肝出血综合征患鸡凝血和纤溶功能的检测分析  

Detection and Analysis of Coagulation and Fibrinolysis in Experimental Chicken Model of Fatty Liver Hemorrhagic Syndrome

作　　者：姜锦鹏[1] 闻爱友[1] 李磊[1] JIANG Jinpeng;WEN Aiyou;LI Lei(Anhui Province Key Laboratory of Animal Nutritional Regulation and Health,College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院动物科学学院安徽省动物营养调控与健康重点实验室,安徽凤阳233100

出　　处：《安徽科技学院学报》2022年第5期1-6,共6页Journal of Anhui Science and Technology University

基　　金：安徽省自然科学基金(11040606M88);安徽科技学院兽医学重点学科基金(AKZDXK2015A04)。

摘　　要：目的:检测分析雌激素诱导的鸡实验性脂肪肝出血综合征发生、发展过程中凝血功能和纤溶功能的变化。方法:96只29日龄817肉杂鸡随机分为4组。Ⅰ~Ⅲ组分别肌肉注射30、300、3000μg/kg·bw苯甲酸雌二醇,对照组(C组)肌肉注射等体积的玉米油。试验期4周。试验期间测定鸡的脂肪沉积、肝脏出血程度、血块收缩指数、血钙、血磷以及凝血功能和纤溶功能等指标。结果:肌注雌激素4周后,Ⅰ~Ⅲ组的脂肪沉积和肝出血分数有不同程度增加;Ⅰ~Ⅲ组血块收缩指数在雌激素处理1~4周后均有所升高,且有随着雌激素水平升高而增加的趋势;肌注雌激素2或4周后,Ⅱ和Ⅲ组的血浆总钙、离子钙、无机磷含量和碱性磷酸酶活力提高,Ⅰ~III组PT和TT缩短,而血浆Fbg和D-D浓度升高。结论:FLHS患鸡存在凝血及继发性纤溶功能增强,且出现较早并随着雌激素水平增加愈发明显。Objective:To detect and analyze the changes of coagulation function and fibrinolytic function during the occurrence and development of experimental chicken fatty liver hemorrhagic syndrome induced by estrogen.Methods:Ninety-six 29-day-old 817 hybrid broilers were randomly divided into 4 groups.GroupⅠ-Ⅲwere intramuscularly injected with 30,300 and 3000μg/kg·bw estradiol benzoate,respectively.Control group(Group C)was intramuscularly injected with equal volume of corn oil.The experiment lasted for 4 weeks.During the experiment,fat deposition,degree of liver bleeding,blood clot contraction index,blood calcium,blood phosphorus,coagulation function and fibrinolysis function were measured.Results:Four weeks after intramuscular injection of estrogen,the fat deposition and liver hemorrhage score were increased in GroupⅠ-Ⅲ.The blood clot contraction indexes in GroupⅠ-Ⅲwere raised treated while with estrogen for 1-4 weeks,and there was a tendency to increase with estrogen level.After estrogen was administered intramuscularly for 2 or 4 weeks,the contents of plasma total calcium,ionic calcium and inorganic phosphorus and alkaline phosphatase activity in GroupⅡandⅢwere increased,meanwhile,PT and TT were shortened whereas the concentrations of plasma Fbg and D-D were raised in GroupⅠ-Ⅲ.Conclusion:Coagulation and secondary fibrinolysis were enhanced in the chickens with FLHS,which appeared earlier and became more obvious with the increase of estrogen level.

关 键 词：鸡 脂肪肝出血综合征(FLHS) 雌激素 凝血功能 纤溶功能 

分 类 号：S852.3[农业科学—基础兽医学]