乙酰阿魏单硝酸异山梨醇酯对烧伤大鼠皮肤细胞凋亡进程的影响  

Effect of isosorbide mononitrate of acetyl ferulate on apoptosis of skin cells in burned rat

作　　者：王傑 苏福增[1] 刘小龙[1] 姚俊杰 查天建[1] WANG Jie;SU Fuzeng;LIU Xiaolong;YAO Junjie;ZHA Tianjian(Xinjiang Uygur Autonomous Region People's Hospital,Urumqi 830000,China)

机构地区：[1]新疆维吾尔自治区人民医院,乌鲁木齐830000

出　　处：《新疆医科大学学报》2022年第11期1269-1273,共5页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区人民医院院内项目(201810106)。

摘　　要：目的探讨乙酰阿魏单硝酸异山梨醇内酯(Acetyl ferulaic isosorbide,AFI)对实验大鼠烧伤后局部细胞坏死进程的影响。方法实验雄性Wistar大鼠30只,平均体重(200±20)g,麻醉后用10%的硫化钠溶液脱除大鼠背部皮毛,脱毛24 h后,制作深Ⅱ°烧伤模型。根据不同的处理,分为假伤组、及时恢复组、及时复苏+AFI药物组、延迟复苏组以及延迟复苏+AFI药物组,检测各组大鼠血清和皮肤组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)和一氧化氮合酶(NO合酶)含量变化。结果(1)假伤组大鼠血清、皮肤SOD活性均高于烧伤各组大鼠(P<0.05);使用AFI药物的及时复苏组大鼠与延迟复苏组大鼠血清、皮肤SOD活性均高于未使用AFI药物组大鼠(P<0.05)。(2)烧伤各组大鼠血清、皮肤MDA水平相比于假伤组均显著增加(P<0.05);进行复苏处理的大鼠中,使用AFI治疗干预的大鼠血清、皮肤MDA水平与未使用AFI治疗的大鼠相比显著降低(P<0.05)。(3)烧伤各组大鼠血清、皮肤NO和NO合酶含量相比于假伤组均显著增加(P<0.05);进行复苏处理的大鼠中,使用AFI治疗干预的大鼠血清、皮肤NO和NO合酶含量与未使用AFI治疗的大鼠相比显著降低,差异均有统计学意义(P<0.05)。结论AFI能有效控制实验大鼠烧伤后表皮细胞的凋亡进程,其功能机理与提高细胞抗氧化酶活力和通过消除氧自由基控制脑组织的过氧化过程相关。Objective To investigate the effect of Acetyl ferulaic isosorbide mononitrate(AFI)on the process of local cell necrosis in experimental burned rats.Methods 30 male Wistar rats,with an average body weight of(200±20)g,were anaesthetized and stripped of the dorsal fur with 10%sodium sulphide solution for 24 h.A deep II°burn model was prepared.The rats were divided into sham injury group,prompt recovery group,prompt recovery+AFI drug group,delayed recovery group and delayed recovery+AFI drug group according to the different treatments,and the changes of superoxide dismutase(SOD),malondialdehyde(MDA),nitric oxide(NO)and nitric oxide synthase(NO synthase)contents in the serum and skin tissues of each group were measured.Results(1)SOD activities in serum and skin of the rats in sham injury group were higher than those in burn group(P<0.05);SOD activities in serum and skin of the rats in timely resuscitation group and delayed resuscitation group treated with AFI were significantly higher than those in rats not treated with AFI(P<0.05).(2)MDA levels in serum and skin of the rats in burn group were significantly increased compared with the sham injury group(P<0.05);The serum and skin MDA levels of the rats treated with AFI were significantly lower than those of the rats not treated with AFI(P<0.05).(3)Compared with sham injury group,the contents of NO and NO synthase in serum and skin of the rats in burn group were significantly increased(P<0.05);In the resuscitation rats,the serum and skin NO and NO synthase contents of the rats treated with AFI were significantly lower than those of the rats not treated with AFI(P<0.05).Conclusion AFI can effectively control the apoptotic process of epidermal cells after burns in rats,and its functional mechanism is related to the enhancement of cellular antioxidant enzyme activity and the control of peroxidation in brain tissue by eliminating oxygen free radicals.

关 键 词：烧伤 皮肤细胞凋亡 乙酰阿魏单硝酸异山梨醇酯 过氧化 氧化应激 

分 类 号：R644[医药卫生—外科学]